Competition:Analysis of Data:Sample Experiment:% of Control

Analysis of Data as Percent of Control Versus - Log Prazosin

On this page you will convert the data to % of Control and estimate the IC₅₀ value from a plot of % of Control versus -Log Prazosin.

Matthew has placed you calculations from the previous table in the table below

You now need to calculate the % of Control.  

• The control value would be the amount bound in the absence of unlabelled ligand, the highest number in the data set or the top of the curve defined by nonlinear regression analysis 
• To calculate the % of control, all of the values needed to be divided by the control value and converted to percent.

Calculate the % of control now and enter the results in the last column.  Use the value obtained in the absence of prazosin (Log -12) as the control value.

Prazosin Log (M)	Bound (cpm)	% of Control
- 12.00	1395
- 9.52	1292
-9.00	1256
-8.52	958
-8.00	730
-7.52	417
-7.00	198
-6.52	126
-6.00	116
-5.52	82
-5.00	81

The plot of % of Control versus Log of the Prazosin Concentration looks like this:

Continue to analyze the data as percent of Specific Bound vs. - Log Prazosin