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Biochemistry and Molecular Biology
Pi-Wan Cheng, Ph.D.
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ACADEMIC DEGREES:
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Graduate School: |
Ph.D. in Biochemistry
(1975)
Case Western Reserve University School of Medicine
Cleveland, OH |
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Post-doctoral Positions: |
Department of
Pediatrics (Pulmonary Division)
Case Western Reserve University |
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Contact Name: Pi-Wan Cheng, Ph.D., Professor
Biochemistry and Molecular Biology
Office: 7042
Durham Research Center
Phone Number: 402-559-5776
e-mail address: pcheng@unmc.edu
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Agency: Nebraska Research Initiative
Title: Cancer Glycobiology
Role on Project: PI (10% effort)
Dates & total direct cost: 07/01/06-06/30/10; $1,100,000
Agency: NIH RO1
HL48282-10
Title: Biosynthesis of Tracheal Mucous Glycoproteins
Role on Project: PI (40% effort)
Dates & direct cost of project: 08/01/08 ‑ 07/31/09; $250,000
Agency: Nebraska Department of Health LB506
Title: Role of secreted mucins in
suppression of tumorigenicity
Role on Project: PI (5% effort)
Dates & total direct cost: 07/01/08-06/30/09; $40,000
Agency: US Army PC074289
(PI: M-F Lin)
Title: Aberrant expression of protein
phosphatases during androgen-independent
growth in human prostate cancer cells
Role on Project: PI (3% effort)
Dates & total direct cost: 4/1/08-3/31/11; $700,000
Agency: US Army
Postdoctoral Fellowship (PI: Prakash Radhakrishnan)
Title: Role of L-selectin
and sialyl Lewis antigens in prostate cancer metastasis
Role on Project: Mentor (5% effort)
Dates & total direct cost: 04/01/08-03/31/10; $90,000
Agency: Nebraska
Department of Health LB506
Title: Mucin Glycans as Prognostic Markers of Lung Cancer
Role on Project: PI (5% effort)
Dates & total direct cost: 07/01/07-06/30/08; $40,000
Agency:
Nebraska Department of Health LB506
Title: Roles of mucin glycans in mammary cancer metastasis
Role on Project: PI (5% effort)
Dates & total direct cost: 07/01/06-06/30/07; $40,000
Agency: Nebraska Department of Health LB506
Title: Gene Therapy of Lung Cancer
Role on Project: PI (5% effort)
Dates & total direct cost: 07/01/05-06/30/06; $40,000
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Beum P, Bastola DR, and Cheng P-W: Mucin
biosynthesis: EGF downregulates core 2 enzymes in a human airway
adenocarcinoma cell line. Am. J. Respir. Cell and Mol. Biol.
29-48-56, 2003.
Choi, K., Osorio, F., and Cheng, P.-W.: Mucin
biosynthesis: C2GnT-M gene, tissue-specific expression, and bovine herpes
virus-4 homologue. Am. J. Respir. Cell and Mol. Biol. 30:1-10, 2004.
Amrite, A.C., Cherruvu, N.P.S., Sundaram, S.,
Aravalli, R., Cheng, P.-W., and Kompella, L.B.: Lung Gene Therapy: Clinical
and Regulatory Issues. Clinical Res. and Regulatory Affairs 21:1-28,2004.
Beum, P.V., Basma, H., Bastola, D.R., and
Cheng, P.-W.: Mucin biosynthesis: upregulation of core 2b1,6
N-acetylglucosaminyltransferase by retinoic acid and Th2 cytokines in a
human airway epithelial cell line. J. Cell Phyciol.-Lung Cell. and Mol.
Physiol. 288:L116-L124, 2005.
Singh, J., Khan, G., Kinarsky, L., Choi, K., Cheng, H., Wilken, J., Bedows,
E., Sherman, S., and Cheng, P.-W.: Identification of disulfide bonds among
the nine core 2 N-acetylglucosaminyl-transferase-M cysteines conserved in
mucin
b6
N-acetylglucosaminyltransferase family. J. Biol. Chem. 279:38969-77, 2004.
Choi, K.H., Basma, H., Singh, J. and
Cheng. P.-W. Enhancement of the expression of CMV promoter-controlled
glycosyltransferase and
b-galactosidase
transgenes by butyrate, tricostatin A, and 5-aza-2’-deoxycytidine.
Glycoconjugate J. 22:63-9, 2005.
Bandi, N., Ayalasomayajula,
S.P., Iwakawa, J., Cheng, P.-W., and Kompella, U.B. Intratracheal
Budesonide-poly(lactide-co-glycolide) Microparticles Ameliorate Early
Biochemical Changes in a Benzo(a)pyrene-fed Mouse Model. Journal of Pharmacy
and Pharmacol. 57:851-60, 2005.
Basma*, H, El-Refaey*, H,
Sgagias, MK, Cowan, KH, Luo, X, and Cheng, P-W: Bcl-2 antisense enhances
cisplatin-induced apoptosis in isogenic MCF-7 breast cancer lines with and
without functional p53. J. Biomed. Sci. 12:999-1011, 2005. (*, Equal
contribution)
Tan, S and Cheng, P-W: Mucin
biosynthesis: identification of the cis-regualtory elements of human C2GnT-M
gene. Am. J. Respir. Cell and Mol. Biol. 36:737-45, 2007.
Radahkrishnan R, Beum P, and
Cheng P-W: Butyrate induces the synthesis of sialyl Lewis x carbohydrate
epitope in a pancreatic adenocarcinoma cell line. Biochem Biophys Res Commun.
359:457-62, 2007.
Hashimotto M, Tan S, Mori N,
Cheng H, and Cheng P-W: Mucin biosynthesis: Molecular cloning and expression
of mouse mucus-type core 2 β1,6 N-acetylglucosaminyltransferase.
Glycobiology 17(9):994-1006, 2007.
Radhakrishnan P and Cheng P-W.
GCNT3 (glucosaminyl (N-acetyl) transferase 3, mucin type). Atlas Genet
Cytogenet Oncol Haematol. November 2007 URL: http://AtlasGeneticsOncology.
org/ Genes/ GCNT3 ID44105ch15q21.html
Radhakrishnan, P*, Basma, H*,
Klinkebiel, D, Christman, J, and Cheng, P-W: Cell type-specific activation
of the cytomegalovirus promoter by dimethylsulfoxide and
5-aza-2’-deoxycytidine. Int. J. Biochem and Cell Biol. 40: 1944-55, 2008.
doi:10.1016/j.biocel.2008.02.014. (*, equal contribution)
Radhakrishnan P, Lin M-F, and Cheng P-W: Elevated expression of L-selectin
ligand in lymphoid node-derived human prostate cancer cells correlates with
increased tumorigenicity. Glycoconjugate J (in press) DOI:
10.1007/s10719-008-9167-z
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PREVIOUS
GRADUATE STUDENTS/POST-DOCTORAL FELLOWS (present location):
Stuart Levin, Medical Student at
University of North Carolina at Chapel Hill, won the first prize in
medical student research presentation competition with the work done in
my laboratory.
James Sherman, M.D., Professor and Chief of Pediatric Pulmonology, Univ.
of Florida at Gainsville.
Margaret Leigh, M.D., Professor and Chief of Pediatric Pulmonology, UNC-Chapel
Hill.
Michael Konstan, M.D., Professor of Pediatric Pulmonology, Case Western
Reserve University.
William Wingert, Ph.D., Clinical Chemist, Trenton, New Jersey.
Philip Ropp, Ph.D., Biochemist, Durham, North Carolina.
Cheng-Ming Li, Ph.D., Biochemist, Bayer Company, Research Triangle Park,
NC.
Paul Beum, Ph.D., Postdoctoral Fellow, University Of Virginia,
Charlottesville, VA.
Katsunori Yanagihara, MD-PhD, Assistant Professor, Nagasaki University,
Japan.
Masafumi Seki, MD-PhD, Assistant Professor, Nagasaki University, Japan.
Jun Iwakawa, MD-PhD, Assistant Professor, Nagasaki University, Japan.
Hashimoto Mitsuyoshi, MD-PhD, Associate Professor, Nagoya University,
Japan.
Hesham Basma, Ph.D., Research Associate, Department of Surgery, UNMC.
Kyung Choi, Ph.D., Postdoctoral Research Associate, Eppley Institute,
UNMC
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Our lab is studying the
structure and function of glycoconjugates in health and disease. We try to
manipulate the carbohydrate structures by modulating the expression of some
key glycosyltransferases through over-expression, knockdown, or redirecting
Golgi localization of these enzymes and then monitoring the effects of these
manipulations on selectin binding and metastasis of cancer cells. Other
projects include biochemical and X-ray crystal structural characterization
of glycosyltransferases, regulation of the expression of glycosytransferase
genes, and employment of lectin or carbohydrate for gene targeting.
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None
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