Pathology and Microbiology

John D. Jackson, Ph.D.

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ACADEMIC DEGREES:

Undergraduate School	B.S., University of North Carolina
Graduate School:	M.S., University of Nebraska Medical Center Ph.D., University of Nebraska Medical Center
Post-doctoral training:	DNAX Research Institute, Palo Alto, CA

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Contact Name: John D. Jackson, Ph.D
Phone Number: 402-559-7623
e-mail address: jdjackso@unmc.edu

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• GRANT SUPPORT (last 3 years):

Principal Investigator, Nebraska Department of Health (LB506), “Optimizing Cytokine Mobilization of Antitumor Autografts.”  July 1, 2002 – June 30, 2003 ($39,318).

Co-Investigator, NIH RO1 (Principal Investigator, Dr. Stephen Rennard), “Stem Cells for the Prevention and Treatment of Emphysema”. August 1, 2003 – June 30, 2007 ($1,000,000).

Co-Investigator, NIH NIA RO1 (1R01AG024912-01) (Principal Investigator, Dr. Graham Sharp) “Stem Cell Quality Assays: Correlation with Aging/Health”, September 30, 2004 – July 31, 2009.

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• PUBLICATIONS (last 3-5 years):

Bhattacharya, S., Jackson, J.D., Das, A.V., Thoreson, W.B., Kuszynski, C., James, J., Joshi, S., Ahmad, I. (2003) Direct identification and enrichment of retinal stem cell/progenitors by Hoechst dye efflux assay.  Invest Ophthalmol Vis Sci, 44:2764-2773.

Sharp, J.G., O’Kane Murphy, B., Jackson. J.D., Brusnahan, S.K., Kessinger, A., Neff, J.R.  (2004) Promises and pitfalls of stem cell therapy for promotion of bone healing.  Clinical Orthopaedics and Related Research, in press.

Zhang, J. L., Cai, J., Jackson, J. D., Kuszynski, C. A., Walls, S., McIvor, R. S. and Fox, I. J. (2005) Long-term transgene expression and survival of transgene-expressing grafts following lentivirus transduction of bone marrow side population cells. Transplantation, 79(8): 882-888.

Zhang, J. L., Cai, J., Jackson, J. D., Walls, S., Kuszynski, C. A. and Fox, I. J. (2005) Competitive equality of donor cells expressing a disparate MHC antigen following stem cell-enriched bone marrow transplantation. Transplantation, 79(10): 1332-1337.

Zhang, J. L., Cai, J., Walls, S., Jackson, J. D., Kuszynski, C. A., Zhao, Y., Pawliuk, R., Leboulch, P. and Fox, I. J. (2005) Tolerance by selective in vivo expansion of foreign major histocompatibility complex-transduced autologous bone marrow. Transplantation, 80(3): 362-369.

Kessinger, A., O'Kane Murphy, B., Jackson, J. and Sharp, J. (2005) An ex vivo model of hematopoietic stem cell mobilization. Cytotherapy, 7(6): 463-469.

Jackson, J.D., Sharp, J.G., Haider, H., Garvin, K.L. and Namavar, F. (2006) Preliminary analysis of attachment, survival and growth of bone marrow stromal cells on nanocrystalline hard ceramic coatings. In:  Materials for Scaffolding of Biologically Engineered Systems: Interfaces and Interactions on a Nanoscale, (Ravaglioli, A. and Krajewski, A, eds), Consigloi Nazionale Delle Ricerche, Rome, Italy, pp. 109-118.

Floreani, A.A., Gunselman S.J., Heires, A.J., Hauke, R.J., Tarantolo, S. and Jackson, J.D. (2007) Novel C5a agonist-based dendritic cell vaccine in a murine model of melanoma.  Cell Cycle, 6(22):2835-2839.

Namavar, F., Jackson, J.D., Sharp, J.G., Mann, E.E., Kenneth Bayles, K, Cheung, B.C.L., Feschuk, C.A., Varma, S., Hani Haider, H. and Garvin, K.L. (2007) Searching for smart durable coatings to promote bone marrow stromal cell growth while preventing biofilm formation. In:  Biofilm-Material Interactions-New Tools, Technologies, And Opportunities, (Liberam M., Camesano, T., Kreiswirth, B., Li, P., Richards, R.G., eds), Material Research Society Symposium Proceedings 954E, Warrendale, PA, 0954-H04-04.

Namavar, F., Jackson, J.D., Sharp, J.G., Gustafson, T, Kibuule, L.K., Mann, E.E., Bayles, K, Hani Haider, H. and Garvin, K.L. (2007) Triple smart surfaces for implant devices.  In:  Nanotechnology for Functional Repair and Regenerative Medicine: The Role of Ceramincs as Bulk and as Coating, (Ravaglioli, A. and Krajewski, A, eds), Consigloi Nazionale Delle Ricerche, Rome, Italy, pp.

Sharp, J.G. and Jackson, J.D., (2008) Hematopoietic Stem Cells and Cytokines. In: “Atlas of Clinical Hematology”.  (Armitage, J.O. ed.), Current Medicine Group, Springer Science+Business Media, Philadelphia, pp. 201-222.

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• PREVIOUS GRADUATE STUDENTS/POST-DOCTORAL FELLOWS (present location):

Fernella Hoge, M.S. (Risk Management Specialist, Alegent Health Immanuel Medical Center)

Lisabeth Welniak, Ph.D. (Assistant Professor, University of Nevada School of Medicine)

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• CURRENT RESEARCH PROJECTS:

My research interest involves the study of hematopoietic stem and progenitor cells.

One project concerns the development of a cancer vaccine.  C5a agonists are used to activate dendritic cells and carry a tumor associated antigen into the dendritic cells for processing and presentation to cytotoxic T cells.  Activated dendritic cells as well as activated cytotoxic T cells are used to treat tumor bearing mice to determine the potential of this unique therapy. 

Another project in the laboratory is examining the effects of aging on bone marrow mesenchymal stem cells.  Clonogenic mesenchymal stem cells do not change during aging; however, the consumption of ethanol decreases the number of mesenchymal stem cells presumably through the induction of adipocyte differentiation.  Therefore, as an extension of these findings we have initiated a study to examine the effects of ethanol on mesenchymal stem cell growth and differentiation.    

A third area of research interest involves examining the effects of different nanocrystal ceramic coatings on bone marrow mesenchymal stromal cell growth and differentiation.  Certain nanocrystal coatings of metal oxides such as zirconium dioxide and tantalum pentoxide enhance the attachment and growth of mesenchymal stromal cells.  We are interested in the development of “smart” surfaces on orthopedic prostheses that will promote the attachment and growth of osteoblast precursors (mesenchymal stem cells) to enhance the osteo-intergration of these implants. 

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• SPECIALIZED LABORATORY/CLINICAL RESEARCH RESOURCES:

Stem cell isolation, growth and differentiation
Hematological analysis