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        Obstetrics and Gynecology and Physiology and Biophysics

        Shyamal K. Roy, Ph. D.

            

Year Degree Obtained  Educational Institution
1973 B. Sc. (Physiology)  City College, University of Calcutta, India
1975 M. Sc. (Physiology)  University of Calcutta, India
1980  Ph. D. (Reproductive Physiology) All India Institute of Medical Sciences, New Delhi, India
     
Continued Education  
1987 Certificate in Molecular Biology American Type Culture Collection, (NIH Subsidiary), Rockville, MD.
1993 Certificate in Cell Signaling Life Technologies, Germantown, MD
1998 Educations Workshop UNMC (based on Dean's nomination)

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Contact Name: Shyamal K. Roy, Ph. D., Professor
Interim Director of Reproductive Endocrinology and Infertility
Departments of OB/GYN and Physiology and Biophysics

University of Nebraska Medical Center
Phone Number:  402-559-6163
Fax Number: 402-559-6164
e-mail address:  skroy@unmc.edu
R01 HD-38468 (S.K. Roy)
NIH/NICHD
Follicular morphogenesis during perinatal development
The goal of this project is to identify molecular mechanism(s) involved in primordial follicle formation in the ovary
Role in Project: PI
07/01/2007 – 6/30/2012
   
P01-HL062222 (I.H. Zucker)
Core C - Molecular Biology, Biochemistry and Histology Core
The goal of this core unit is to provide complementary molecular, cellular, biochemical and scientific support for the four research projects by carrying out a variety of biochemical, molecular biological and histological techniques.
Role in Project: Core Director
07/01/2004-06/30/2009
   
R01-HL66446-05A1 (G. J. Rozanski)
National Institutes of Health
Redox Regulation of Cardiac Electrical Remodeling
This project will explore the electrophysiological function and regulation of the thioredoxin system in controlling the remodeling of K+ channels in the failing heart induced by chronic myocardial infarction.
Role in Project: Co-I
04/01/2006-03/31/2010

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Harvell DME, Strecker TE, Tochacel M, Xie B, Pennigton KL, McComb RD, Roy SK, Shull JD Steckler T, Wang J, Bartol FF, Roy SK, and Padmanabhan V. Fetal programming: Prenatal Testosterone Treatment Causes Intrauterine Growth Retardation, reduces ovarian reserve and increases ovarian follicular recruitment. Endocrinology 146:3185-3193, 2005

Li X, Li S, Xu Z, Lou MF, Anding P, Liu D, Roy SK, Rozanski GJ. Redox control of K+ channel remodeling in rat ventricle. J Mol Cell Cardiol 40:333-4, 2006.

Wang C, Roy SK. Expression of Growth Differentiation Factor 9 in the Oocytes Is Essential for the Development of Primordial Follicles in the Hamster Ovary. Endocrinology 147: 1725-174, 2006.

Yang P, Roy SK. A novel mechanism of FSH regulation of DNA synthesis in the granulosa cells of hamster preantral follicles.  Involvement of a protein kinase C mediated MAP kinase 3/1 self-activation loop. Biol Reprod  75:140-148, 2006.

Yang P, Roy SK. Transforming growth factor B1 stimulated DNA synthesis in the granulosa cells of preantral follicles: Negative interaction with epidermal growth factor. Biol Reprod  75:149-157, 2006.

Liu D, Gao L, Roy SK, Cornish KG, Zucker IH. Neuronal angiotensin II type I receptor upregulation in heart failure: Activation of activator protein 1 and Jun N-terminal kinase. Ciculation Research  99: 1004-1011, 2006.

Wang C, Roy SK. Development of primordial follicles in the hamster: Role of estradiol17b. Endocrinology 148:1707-1716, 2007

Wang C, Prossnitz ER, Roy SK. Expression of GPR30 in the hamster ovary: differential regulation by gonadotropins and steroid hormones. Endocrinology 148: 4853-4864, 2007.

Wang C, Roy SK. Hormonal regulation of bone morphogenetic protein  receptor (BMPR) expression in perinatal hamster ovary, and the effect of BMPs on the formation and development of primordial follicles. Biol Reprod (Suppl), Abstract 280, 2007.

Hou X, Arvisais EW, Jiang C, Chen DB, Roy SK, Pate JL, Hansen TR, Rueda BR and Davis JS. Prostaglandin F2α (PGF2α) Stimulates the Expression and Secretion of TGFB1via induction of the Early Growth Response 1 Gene (EGR1) in the Bovine Corpus Luteum. Mol Endocrinol 22:403-414, 2007

Liu D, Gao L, Roy SK, Cornish KG, Zucker IH. Role of Oxidant Stress on AT1 receptor expression in neurons of rabbits with heart failure and in cultured neurons. Circ Res 103: 186-193, 2008

Wang C, Prossnitz ER and Roy SK. G protein-coupled receptor 30 expression is required for estrogen stimulation of primordial follicle formation in the hamster ovary.  2008 Endocrinology 149: 4452-4461, 2008.

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  • PREVIOUS GRADUATE STUDENTS/POST-DOCTORAL FELLOWS (present location):

Summer Student -- 1990 -- Christine L. Ogren, Buena Vista College, Iowa (recipient of the UNMC Alumni Summer Fellowship)-completed MD

Summer Student -- 1991-- Andrew Kouba, Northwest Missouri College, MO (selected to present his research in the National Undergraduate Research Forum held in March 1992 in Minnesota)- received Ph. D. in Reproductive Biology from University of Florida at Gainesville and doing post-doctoral training in animal reproduction

Summer Student -- 1993-- Michael Bassik, University of Wisconsin, received Ph. D. in Biochemistry and Molecular Biology from University of Wisconsin, and doing post-doctoral training

Summer Student -- 1994-- Delores Terada, Creighton University, NE, Pharmacy graduate from Creighton University, NE, practicing pharmacist.

Summer Student -- 1994-- Sonal Sabena, High School senior, Nebraska, MD; in residency training.

Resident Research Fellow -- 1992-- Ann Sullen, MD, OB/GYN, recipient of the Ortho Award for best research paper in Resident Day, OB/ GYN, practicing Physician in Omaha

Resident Research Fellow – 1997-2000—Brittani Antisdel, MD, OB/GYN, practicing Physician

M. S. Student -- Ni Yu -- Biology (UNO)—completed in March 1998, Working in a Biotech Company in San Francisco

Ph. D. Student-- James Schwartz --- OB/GYN (MSIA)-completed in 2000, In Vitro Fertilization Clinic Director in St. Louis

Ph. D. Student— Yi-Ming Zhang —Post-doctoral research fellow in a laboratory at the University of California, Davis.

Post-doctoral Research Associate -- Alok R. Kole, Ph. D., University of Calcutta, India; Associate professor, Department of Physiology, A.M. College, Calcutta, India

Post-doctoral Research Associate –1999-present- P. Yang, Ph. D., Zhejiang University, P. R. China, Research Associate, OB/GYN, UNMC. Dr. Yang is an Assistant Professor position in the department of Internal Medicine, University of Arkansas Medical Center.

Post-doctoral Research Associate –2001-2004- J. R. Wang, Ph. D., Zhejiang University,    P. R. China. Presently, Post-doctoral Research Associate, Baylor College of Medicine

Summer Student – 1999—Kristina Garnett—University of Nebraska at Kearney, M4 student, UNMC.

Summer student-2000-Amy Guminski-University of Nebraska at Lincoln. Presently an M1 student

Summer student - 2003 - Helene Gougeon - University of Poitiers, France.  Senior year student.

Summer student-2000-Micelle Dorsey, Nebraska Wesleyan University, Lincoln. Presently a graduate student

Summer student-2004-Lindsay Ackerman, UNO. Presently a college senior

Post-doctoral Research Associate–2004-present C. Wang, Ph. D., Zhejiang University, P. R. China

Post-doctoral Research Associate–2008-present Liying Geng, Ph. D., Zhejiang University, P. R. China

Summer student-2006-Jean Huff, M4 Student, UNMC

Summer student-2007-Kristina Derrick, M3 Student, UNMC

Summer student-2008-Chelsea Newman, M2 Student, UNMC

Graduate student (Rotation)-Che Qi- Department of Cellular and Integrative Physiology, UNMC. Presently doing residency program

Graduate student (Rotation)-Jennifer Green- Department of Cellular and Integrative Physiology, UNMC. M4, UNMC

Ph. D. Student—Anindit Mukherjee --- Department of Cellular and Integrative Physiology, 2007-present.

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 Regulation of EGF and TGF-b receptor gene expression in ovarian follicular cells

Mechanisms of gonadotropins and growth factor regulation of cell proliferation in preantral follicles

Factors and mechanisms regulating follicular morphogenesis during ovarian development

Regulation of FSH-receptor gene expression in ovarian cells

Identification of novel genes involved in follicular development

Role of estrogen in folliculogenesis

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Isolation technique and organ culture of intact preantral follicles

Organ culture of intact fetal ovaries

Real time, RT-PCR evaluation of mRNA expression

Immunofluorescence histochemistry

In-situ hybridization of mRNA

Immunoblotting and quantification of growth factor receptors

Kinase assays

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