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Internal Medicine
Pulmonary, Critical Care, Sleep and Allergy Section

Todd A. Wyatt, Ph.D.
Assistant Professor

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ACADEMIC DEGREES:
BS 1986, Vanderbilt University (Biology)
PhD 1992, University of North Carolina at Chapel Hill (Pathology)
Postdoctoral 1995, Vanderbilt University Medical Center (Physiology)

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Contact Name: Todd Wyatt
Phone Number: 402-559-3817
e-mail address: twyatt@unmc.edu)
or Jeanette Danielsen (402-559-9101)

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Agency:

Veterans Affairs Administration Medical Center
VA Merit Review

Title: Malondialdehyde-Acetaldehyde Signaling in Airway Epithelium
Role: Principal Investigator
Date:    10/1/02-9/30/06
Direct Costs: $497,700
   
Agency: American Lung Association
Career Investigator Award
Title: PKC Epsilon: A Regulator of Ciliated Bronchial Epithelial Cell Attachment
Role: Principal Investigator
Date:    7/1/03-6/30/06
Direct Costs: $150,000
   
Agency: NIH-NIAAA
R-01
Title: Ethanol-Mediated Cilia Motility Dysfunction
Role: Co-Principal Investigator (Sisson, PI)
Date:    7/1/00-6/30/11
Direct Costs: $1,174,420
   
Agency: NIH-NHLBI R21ES0113856-01
(R-21, PI Rennard)
Title: Fetal Smoke Exposure and Emphysema: Role of Stem Cells
Role: Co-Investigator
Date: 4/1/2005-3/31/08
Direct Costs: $300,000
   
Agency:

Veterans Affairs Administration Medical Center
 (VA Merit Review, PI- Debra Romberger)

Title: Organic dust epithelial PKC activation & airways disease
Role: Co-Intestigator
Date: 10/1/05-9/30/09
Direct Costs: $685,000
   

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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Wyatt, T. A. and J. H. Sisson. Chronic Ethanol Down-Regulates PKA Activation and Ciliary Beating in Bovine Bronchial Epithelial Cells. Am J Physiol Lung Cell Mol Physiol., 2001, 281:L575-L581.

Thiele, S. Worrall, G.M., D.J. Tuma, L.W. Klassen, T.A. Wyatt, and N. Nagata. The Chemistry and Biologic Effects of Malondialdehyde-
Acetal
dehyde (MAA) Adducts. Alcoholism: Clin Exp Res 25(5): 218S-224S, 2001.

Kashyap, R. A.A.Floreani, A.J.Heires, S.D.Sanderson, and T.A.Wyatt. Protein Kinase C-alpha Mediates Cigarette Smoke Extract and C5a Stimulated IL-8 Release in Human Bronchial Epithelial Cells. J. Investig. Med. 50:46-53, 2002.

Wyatt, T.A., K.K. Kharbanda, D.J. Tuma, and J.H. Sisson. Malondialdehyde-Acetaldehyde-adducted bovine serum albumin activates protein kinase C and stimulates interleukin-8 release in bovine bronchial epithelial cells. Alcohol 25:159-166, 2002.

Kharbanda, K. K., K. A. Shubert, T. A. Wyatt, M. F. Sorrell, and D. J. Tuma. MAA-Adducts Increase Secretion of Urokinase-Like Plasminogen Activator by Hepatic Stellate Cells Via a PKC-dependent Pathway. Biochem. Pharmacol. 63: 553-562, 2002.

Spurzem, J. R., T. Veys, S. I. Rennard, and T. A. Wyatt. Differential Effects of Isoproternol on the Acceleration of Bovine Epithelial Cell Migration. Am J. Physiol., 282: L1108-16, 2002.

Romberger, D. J., V. Bodlak, S. G. VonEssen, T. Mathisen, and T. A. Wyatt. Hog Barn Dust Extract Stimulates IL-8 Release in Human Bronchial Epithelial Cells via PKC Activation. J. Applied Physiol. 93:289-96, 2002.

Koyama, T., T. A. Wyatt, X. Liu, F. Wen, T. Kobayashi, Q. Fang, H. J. Kim, and S. I. Rennard. PGD2 Modulates Fibroblast-mediated Native Collagen Gel Contraction. Am. J. Respir. Cell Mol. Biol. 27:375-381, 2002.

Wyatt, T.A., J. H. Sisson, M. A. Forgèt, R. G. Bennett, F. G. Hamel1, and J. R. Spurzem. Relaxin Stimulates Bronchial Epithelial Cell PKA Activation, Migration and Ciliary Beating. Exp Biol Med. 227:1047-1053, 2002. 

Floreani, A.A., T. A. Wyatt, J. Stoner, S. D. Sanderson, E. G. Thompson, A. J. Heires. Smoke and C5a Induce Airway Epithelial ICAM-1 and Cell Adhesion. Am J Respir Cell Mol Biol 29:472-482, 2003. 

Sisson, J. H., J. A. Stoner, B. A. Ammons and T. A. Wyatt. All-Digital Image Capture and Analysis of Ciliary Beat Frequency. J. Microscopy 211:103-111, 2003.

Lamontagne, N.D., Digital video and software allow whole-field analysis of cilia. Biophotonics Int., 10:26-28, 2003 (article about ref. 27).

Wyatt, T. A., M. Forgèt, and J. H. Sisson. Ethanol Stimulates Ciliary Beating by Dual Cyclic Nucleotide Kinase Activation in Bovine Bronchial Epithelial Cells. Am. J. Path. 163:1157-1166, 2003. 

Forget, M.A., J.H. Sisson, J. R. Spurzem, and T.A. Wyatt. Ethanol increases phosphodiesterase 4 activity in bovine bronchial epithelial cells. Alcohol, 31:31-38, 2003. 

Allen-Gipson, D. S., D. J. Romberger, M. A. Forget, K. L. May, J. H. Sisson, and T. A. Wyatt. IL-8 Inhibits Isoproterenol-Stimulated Ciliary Beat Frequency in Bovine Bronchial Epithelial Cells. J. Aerosol. Med., 17(2):107-115, 2004. 

Mathisen, T., S. G. Von Essen, T. A. Wyatt, D. J. Romberger. Hog barn dust extract augments lymphocyte adhesion to airway epithelial cells. J. Applied Physiol., 96:1738-1744, 2004. 

Fang, Q., X. Liu, S. Abe, T. Kobayashi, X.Q. Wang, T. Kohyama, M. Hashimoto, T. A. Wyatt, S. I. Rennard . Thrombin Induces Collagen Gel Contraction through PAR-1 activation and PKC-e. Eur. Respir. J., 24:918-924, 2004.

Kharbanda, K. K., D. D. Rogers, T. A. Wyatt, M. F. Sorrell, D. J. Tuma. Transforming growth factor-b induces contraction of activated hepatic stellate cells. J Hepatology, 41:60-66, 2004. 

Wyatt, T.A., M. J. Gentry-Nielsen, J. A. Pavlik and J. H. Sisson. Desensitization of PKA-stimulated ciliary beat frequency in an ethanol-fed rat model of cigarette smoke exposure. Alcohol Clin. Exp. Res., 28:998-1004, 2004. 

Wyatt, T. A.,  Forgèt, M.A., J. M. Adams and J.H. Sisson. Both cAMP and cGMP are required for maximal ciliary beat stimulation in a cell-free model of bovine ciliary axonemes. Am J Physiol. (In Press). 

Lambert G. P., J. R. Spurzem, D. J. Romberger, T. A. Wyatt, E. Lyden, A. M. Stromquist, J. A. Merchant, S. Von Essen.  Tumor Necrosis Factor-a Hyper-Responsiveness to Endotoxin in Whole Blood is Associated with Chronic Bronchitis in Farmers. J Agromedicine, 10 (1):39-44, 2005. 

Spurzem, J.R., T. Veys, J. Devasure, J. H. Sisson, and T. A. Wyatt. Ethanol treatment reduces bovine bronchial epithelial cell migration. Alcohol Clin Exp Res. 29: 485–492, 2005. 

Allen-Gipson, D. S., T. A. Wyatt, A. J. Heires, S. D. Sanderson, R. G. MacDonald, A. A. Floreani. Cigarette Smoke Extract increases C5a Receptor expression in Human Bronchial Epithelial cells. J Pharm Exp Ther 314:476, 2005. 

Sisson, J.H., J.A. Stoner, D.J. Romberger, J.R. Spurzem, T. A. Wyatt, J. Owens-Ream, and D.M. Mannino. Alcohol intake may prevent lung restriction. Alcohol, 36:19-30, 2005. 

Vander Top, E. A. T. A. Wyatt, and M. J. Gentry-Nielsen. Smoke Exposure Exacerbates an Ethanol-Induced Defect in Mucociliary Clearance of Streptococcus pneumoniae. Alcohol Clin Exp Res. 29:882–887, 2005. 

Sisson J. H., T. A. Wyatt, Guidot D. M., Bagby G. J., Helander A., Tønnesen H., Spies C. D. Bench-to-bedside: Mechanisms and consequences of alcohol-altered host defenses. Alcohol Clin. Exp Res. 29(6):1090-1097, 2005. 

Wyatt, T. A., K. K. Kharbanda, D. J. Tuma, J. H. Sisson and J. R. Spurzem. Malondialdehyde-acetaldehyde adducts decrease bronchial epithelial wound repair. Alcohol 36:31-40, 2005. 

Vent, J., T. A. Wyatt, D. D. Smith, A. Banerjee, R. F. Ludueña, J. H. Sisson and R. Hallworth. Functional Requirement for Specific b Tubulin Isotypes in Bovine Cilia. J. Cell Sci. 118:4333-4341, 2005. 

Allen-Gipson, D. S., J. Wong, J. R. Spurzem, J. H. Sisson, T. A. Wyatt. Adenosine A2A Receptors Promote Adenosine-stimulated Wound Healing in Bronchial Epithelial Cells. Am. J. Physiol. (In Press), 2006. 

Elliott, M. K., J. H. Sisson, W. W. West, and T. A. Wyatt. Differential in vivo effects of whole cigarette smoke exposure versus cigarette smoke extract on murine ciliated tracheal epithelium. Exp Lung Res (In press), 2006.

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  • PREVIOUS GRADUATE STUDENTS/POST-DOCTORAL FELLOWS (present location):

STUDENTS MENTORED
Undergraduates

Andrew Chu, Princeton University undergraduate, UNMC Summer Research Program 1996
Marc Schultz, Washburn University undergraduate, UNMC Summer Research Program 1997
Alexi Sammut, Colorado State University undergraduate, UNMC (SURP) 2002

Jeremy Nicolarsen, University of Chicago Medical School M2, UNMC (SURP) 2003
Judy Wong, Creighton University Medical School M1, UNMC (SURP) 2003
Karl Spurzem, University of Nebraska-Lincoln undergraduate, UNMC (SURP) 2004
Malorie Messer, Vassar College undergraduate, UNMC (SURP) 2004
Sarah Marshall, UNK undergraduate, INBRE 2005

Medical Students
Susan Schmidt, UNMC Medical Student, UNMC Summer Research Program 1998
Jennifer Mommsen-Adams, UNMC Medical Student, UNMC Summer Research Program 1999

Pulmonary Fellows
Bogdan Nowakowski, MD, UNMC Pulmonary Fellow, Fellowship Research Training Program 1996-1998
Ravinder Shergill, MD, UNMC Pulmonary Fellow, Fellowship Research Training Program 1997-1999
Ravindra Kashyap, MD, UNMC Pulmonary Fellow, Fellowship Research Training Program 1998-2001
Athir Merogi, MD, UNMC Pulmonary Fellow, Fellowship Research Training Program 1999-2002

Nanjappa Somanna, MD
, UNMC Pulmonary Fellow, Fellowship Research Training Program 2003-2004

Postdoctoral Fellows
Patrick Lambert, PhD, Univ. Iowa (2000), 2001-02. Currently an Assistant Professor of Exercise Science, Creighton University.
Diane Allen-Gipson, PhD, Univ. Florida A&M (2000), UNMC Research Associate 2001-2004.
Currently an Instructor of Medicine, UNMC.
Sarah Lund, Ph.D., University of Glasgow (1999), UNMC Research Associate, 2002-2005.
Margaret Elliott, Ph.D., Medical University of South Carolina (2002), UNMC Postdoctoral Trainee, 2002-2005. Currently a Senior Researcher, National Animal Disease Control Center.
Rebecca Slager, PhD. Michigan State University (2004), UNMC Postdoctoral Trainee, 2004-current.
Peter Oldenburg, PhD. East Carolina University (2005), UNMC Postdoctoral Trainee, 2005-current.

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Effect of ethanol on ciliary beating in the airways and how this relates to increased lung disease observed in alcoholics.

Role of aldehyde adducted proteins in airway cell production of inflammatory cytokines and how this relates to cigarette smoking –induced COPD.

Regulation of airway epithelial cell attachment by PKC and how this relates to viral infection loss of lung ciliated cells.

Role of nitric oxide formation in airway epithelial cell signaling in environmental/occupational exposure to dusts/toxins in swine confinement facilities.

Effects of common asthma therapeutic agents on the restructuring/remodeling of damaged or wounded airway epithelium with respect to cyclic nucleotide phosphodiesterase signaling.

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Ciliary Beat Frequency Analyzer
Liquid Scintillation counter
Sievers Nitric Oxide Analyzer
Morphometric Wound Healing analysis
ELISA
Western Blotting
Kinase Assay
Flow Cytometry
Phosphodiesterase Assay
Immunofluorescence Microscopy
Buxco small animal lung plethysmograph

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