William G. Chaney

Professor, Biochemistry and Molecular Biology and Eppley Institute

Phone: 402-559-6657 chaney
Fax: 402-559-6650
Email: wchaney@unmc.edu

Education/Training:
Ph.D., Michigan State University, 1979

Research:

Student research opportunities in my lab:
Graduate Students
Medical students, summer research
Undergraduate students, summer research

Research Interests:

Research in this laboratory is centered on the identification and biological functions of cell-surface molecules.

The role of cell surface carbohydrates in tumorigenesis and metastasis of breast cancer is being studied. The influence of the carbohydrates present at the surface of mouse tumor cell lines on the ability of these tumor cells to undergo metastasis is being investigated using techniques of somatic cell genetics, structural analysis of the oligosaccharides present in these cells, and direct assay of the enzymes responsible for complex carbohydrate biosynthesis.

A second area of interest is in the biochemistry and molecular biology of pancreatic cancer. Using a hamster model of human pancreatic cancer, this laboratory has studied the expression of tumor cell specific cell-surface antigens which react with anti-blood group A antibodies. The glycosyltransferase responsible for blood group A formation, and a membrane glycoprotein which is the acceptor for blood group A modification are induced in pancreatic cancer. The blood group A reactive membrane glycoproteins have been purified, and are being cloned from a cDNA library prepared by this laboratory. Future studies will investigate the distribution and function of these proteins in pancreatic cancer in the hamster model and in humans.

Publications:

Hirota, M., P. Pour and W. Chaney (1992) Modification of Blood Group-A Antigen Expression in a Pancreatic Cancer Cell Line (PC-1) by Inhibitors of N-Glycan Processing. J. Cell. Bioc. 50:13-20.

Hirota, M., P. Pour, M. Tempero and W. Chaney (1992) Purification and analysis of Glycoproteins Bearing Blood Group-A Determinants from Nitrosamine-Induced Hamster Pancreatic Cancer. Carcinogenesis 13:1829-1833.

Lu, Y. And W. Chaney (1993) Induction of N-Acetylglucosaminyltransferase V by Elevated Expression of Activated or Proto-Ha-ras Oncogenes. Mol and Cell. Bioc. 122:85-92.

Hirota, M., M. Mogaki, P. Pour and W. Chaney (1993) Glycan Structure of Blood Group-A Antigen in Hamster Normal Tissues and Pancreatic Cancer, Exp. And Mol. Pathol. 58:169-178.

Hirota, M., P. Pour and W. Chaney (1993). UDP-GalNAc:Fuc Alpha, 1-2 Gal Alpha, 1-3 GalNAc Transferase Activity in Hamster Pancreatic Cancers and in Normal Tissues. Carcinogenesis 14:1349-1353.

Lu, Y., J. Pelling and W. Chaney (1994) Expression of Tumor Cell Surface Beta, 1-6 Branched Oligosaccharides and Lung Metastasis. Clin Exp Metastasis 12:47-54.

Mody, R., S. Joshi and W. Chaney (1995) Use of Lectins as Diagnostic and Therapeutic Tools in Cancer. J. Pharmacol and Toxicol. Methods 33:1-10.

Schmied, B.M., Ulrich, A.B., Matsuzaki, H., El-Metwally, T.H., Ding, X., Fernandes, M.E., Adrian, T.E., Chaney, W.G., Batra, S.K., and Pour, P.M. Biologic instability of pancreatic cancer xenografts in the nude mouse. Carcinogenesis, 21(6):1121-1127, 2000.

Books:

W. Chaney, E. Haas, and C. Price. Sequence Analysis Guide, second edition, 2007.

R. MacDonald and W. Chaney. USMLE Roadmap Biochemistry, McGraw-Hill, New York, 2007.

Current Grants and Contracts:

National Institutes of Health P20RR016469
Nebraska Research Network in Functional Genomics
5/1/09 - 4/30/14

Department of Defense
Nebraska Prostate Cancer Research Program
04/15/10 - 03/31/13

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