Confocal Laser Scanning Microscope

The Confocal Laser Scanning Microscope Core Facility operates in the Department of Biochemistry and Molecular Biology on the campus of UNMC. The facility provides instruction in use of the LSM and LSM access for trained investigators on a fee-for-use-time basis. The facility will also collect and process images from samples supplied by investigators on a fee-for-service basis.

All users of the Cell Analysis Facility are obligated to acknowledge the facility in formal publications and presentations which include data generated in the facility.  We also appreciate receiving reprints of such publications.  

Confocal Acknowledgment:  "We thank Janice A. Taylor and James R. Talaska of the Confocal Laser Scanning Microscope Core Facility at the University of Nebraska Medical Center for providing assistance with confocal microscopy and the Nebraska Research Initiative and the Eppley Cancer Center for their support of the Core Facility."

 The facility has a Zeiss 510 Meta Confocal Laser Scanning Microscope equipped with 4 lasers: a Blue Diode 405nm; an Argon Laser 458/477/488 514nm; a DPSS 561nm and a HeNe 633nm. Nomarski optics allow superimposition of signals from the fluorophores overlaid with a DIC image of cells. Zeiss software is available for reconstruction of 3-D images, presentation of image "galleries", Z-sectioning, Orthogonal sectioning, time series, etc. Zeiss software also includes data analysis software for the quantitation of the fluorescence, analysis of colocalization, etc.  Images can be directly stored on CDs or DVDs. The Zeiss Axiovert inverted microscope used by the system has 10x, 20x, 40x oil, 63x oil, and 40x water objectives and a Z-axis motor as well as an xy stage motor. All functions are computer automated with Zeiss Aim software. Controlled environment chambers are available for studies of live cells.

 The facility also has a Zeiss 710 Confocal Laser Scanning Microscope equipped with 4 lasers: a Blue Diode 405 nm; an Argon Laser 458/488/514nm; a DPSS 561nm; and a HENe 633nm. Nomarski optics allow superimposition of signals from the fluorophores overlaid with a DIC image of cells. Zeiss software is available for reconstruction of 3-D images, presentation of image "galleries", Z-sectioning, Orthogonal sectioning, time series, etc. Zeiss software also includes data analysis software for the quantitation of the fluorescence, analysis of colocalization, etc.  Images can be directly stored on CDs or DVDs. The Zeiss Axio observer inverted microscope used by the system has 10x, 20x, 40x oil, 63x oil and a Z-axis motor, as well as an xy stage motor. All functions are computer automated with Zeiss Zen software. The large incubator with heat, CO2 and humidity is available for live cell studies.

Equipment for processing fixed and frozen tissues/cells includes a Shandon 3 Cytospin.

Contact Information
Director: Steve Caplan, Ph.D.
Manager: Janice Taylor, B.S.
Operator: James R. Talaska, B.S. 

1056 Durham Research Center I
Phone: 402-559-6659
Fax: 402-559-6650

Hours of Operation:
8 a.m. - 7 p.m.

Appointments for Consultation and Use:
8 a.m. - 4 p.m.

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