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Pi-Wan Cheng
Professor, Biochemistry and Molecular Biology
Ph.D., Case Western Reserve University, 1975
Phone: (402) 559-5776
Lab: (402) 559-7718
Fax: (402) 559-6650
Email: pcheng@unmc.edu
Click here for biographical sketch and further publications.
Glycobiology is the major research area in my laboratory. We study the functions of mucin-type carbohydrates in health and diseases by a) characterizing the structure of glycosyltransferases, b) studying the regulation of glycosyltransferase genes, and c) identifying roles of mucin glycans in cancer progression and metastasis.
Specific projects are listed as follows:
- Determination of the active site of mucin core 2-M glycosyltranfesrase by X-ray crystallography, mutagenesis, and molecular modeling.
- Regulation of glycosyltransferase genes by characterization of promoter and transcription factors.
- Roles of mucin glycans in tumorigenesis and cancer metastasis.
- Determination of Golgi targeting and retention signals of glycosyltransferases.
RECENT PUBLICATIONS:
Tan S and Cheng PW. Mucin biosynthesis: identification of the cis-regulatory elements of human C2GnT-M gene. Am. J. Respir. Cell and Mol. Biol. 36:737-45, 2007. Abstract
Radhakrishnan P, Beum P, and Cheng PW. Butyrate induces the synthesis of sialyl Lewis x carbohydrate epitope in a pancreatic adenocarcinoma cell line. Biochem. Biophys. Res. Commun. 359:457-62, 2007. Abstract
Hashimoto M, Tan S, Mori N, Cheng H, and Cheng PW. Mucin biosynthesis: Molecular cloning and expression of mouse mucus-type core 2 β1,6 N-acetylglucosaminyltransferase. Glycobiology 17(9):994-1006, 2007. Abstract
Radhakrishnan P and Cheng P-W. GCNT3 (glucosaminyl (N-acetyl) transferase 3, mucin type). Atlas Genet Cytogenet Oncol Haematol. November 2007 URL: http://AtlasGeneticsOncology/org/Genes/GCNT3ID44105ch15q21.html
Radhakrishnan P, Basma H, Klinkebiel D, Christman J, Cheng PW. Cell type-specific activation of the cytomegalovirus promoter by dimethylsulfoxide and 5-aza-2'-deoxycytidine. Int. J. Biochem. Cell. Biol. 40(9):1944-55, 2008. Abstract
Radhakrishnan P, Lin MF, Cheng PW. Elevated expression of L-selectin ligand in lymph node-derived human prostate cancer cells correlates with increased tumorigenicity. Glycoconj J. 26(1):75-81, 2009. Abstract
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