UNIVERSITY OF NEBRASKA–LINCOLN CORE FACILITIES
The University of Nebraska–Lincoln operates the Center for Biotechnology, in the Beadle Center, which provides researchers with access to a wide range of large or sophisticated instrumentation. Core facilities specific to this application are the Protein Core Facility, the Microscopy Core Facility, the Flow Cytometry Core Facility, the Bioinformatics Core Facility and the Nebraska Center for Mass Spectrometry.
The Genomics Core Research Facility is located in UNL's Center for Biotechnology. They provide Illumina/Solexa next generation sequencing and Affymetrix GeneChip DNA microarray services. They also maintain several multi-user imaging systems such as the UVP EPI Chemi II Darkroom, the BioRad Personal Molecular Imager FX and the BioRad iCycler iQ Real Time PCR system.
The flow cytometry core facility, also affiliated with the Center for Biotechnology, provides a broad range of instrumentation as well as consultation services in technical assistance and operation of instruments, data analysis and interpretation of results. The facility houses two flow cytometers, a Becton Dickinson FACvantage Cell Sorter and a Becton Dickinson FACScan that collectively provide for flexibility in the analysis and sorting of cells, organelles and chromosomes. In addition, multicolor experiments as well as measurements of physiological responses of live cells are possible. An automated cell delivery system permits sorting individual cells for direct cloning into 96-well plates and for sorting individual particles. At present, the core facility provides the option of up to 4-color sorting.
The protein core facility, in the Beadle Center, provides standard services related to state-of-the-art protein and peptide analyses including proteomic services, high resolution 2-D gel electrophoresis, imaging, spot picking and digestion, protein sequencing (performed on an ABI Procise-494 automated sequencer), peptide synthesis (performed on an ABI-Pioneer synthesizer. The core facility will also aid in the design, synthesis and conjugation of peptides that can be used as immunogens, substrates or standards, and protein purification.
The microscopy core facility provides instrumentation as well as offering consultation services in technical assistance and operation of instruments, data analysis and interpretation of results. The facility houses a variety of microscopes including advanced fluorescence microscopes (confocal, inverted, upright, stereo) and electron microscopes (transmission, variable pressure scanning, field emission scanning). The wide range of instrumentation permits a variety of studies to be conducted such as advanced immunofluorescence and histochemical analysis of proteins in animal and plant cells, and bacterial and viral pathogenesis; live cell imaging; and morphological, ultrastructural and topographic analysis in biological and chemical materials.
The bioinformatics core facility provides computational support for molecular biology, genomics, proteomics and other biology related disciplines. The facility is equipped with state-of-the-art high performance computer hardware, specialized software, and advanced database systems. The facility provides consultation in experimental design, data analysis, software training and database support services.
The Nebraska Center for Mass Spectrometry is home to six mass spectrometers. These instruments provide a wide range of mass spectrometric analysis. Both low and high resolution data can be obtained in conjunction with electron ionization (EI), and chemical ionization (CI). The Center can also offer electrospray (ESI), matrix-assisted laser desorption (MALDI), and tandem mass spectrometry (MS/MS).
UNL has an up-to-date animal facility that is housed in the School of Veterinary Medicine and Biomedical Sciences. It is responsible for animal procurement, husbandry and veterinary services.
College of Dentistry faculty also work in collaboration with members of the Nebraska Center for Virology and the Department of Special Education and Communication Disorders in The Barkley Memorial Center.
UNIVERSITY OF NEBRASKA MEDICAL CENTER CORE FACILITIES
UNMC facilities are housed in various departments throughout the campus. Many of them are funded by the Nebraska Research Initiative and partially maintained by a fee for service. The facilities are reviewed periodically and support is redistributed and new cores developed as new technologies change.
Microscopy Core Facilities
- Electron microscopy. The electron microscopy core facility is housed in the Department of Genetics, Cell Biology and Anatomy and is equipped with two transmission electron microscopes, a Philips 201 for routine work and a Philips 410 for specialized applications. A JEOL T-220A scanning electron microscope is available and a Philips 515 SEM and the Kevex 7000 EDS x-ray analysis system are also available on campus. The facility is equipped with ultramicrotomes and has the capacity to cut frozen sections at liquid nitrogen temperature for immunocytochemical applications.
- Confocal laser scanning microscopy. The confocal facility is housed in the Department of Biochemistry and Molecular Biology. The facility has a full time dedicated technician who will provide free training so that investigators can use the microscopes themselves on a fee for use time basis. The facility will also collect and process images from samples supplied by investigators on a fee for service basis. The equipment includes a Zeiss Confocal LSM410 equipped with a number of lasers and software for reconstruction of 3-D images Fluorescence in situ hybridization. The Department of Pathology and Microbiology operates a core facility for FISH.
Histology Core Facility
The histology core facility is housed in the Eppley Institute, and overseen by a pathologist with expertise in mouse pathology. The service provides routine histology as well as cutting and processing frozen sections for immunohistochemistry or in situ hybridization.
Molecular Biology Core Facility
The molecular biology core lab is housed in the Eppley Institute and provides two primary services: oligonucleotide synthesis and DNA sequencing.
Monoclonal Antibody Core Facility
The monoclonal antibody core lab is housed in the Eppley Institute and provides a wide range of antibody-related services including hybridoma development, antibody production, purification and characterization. Fees are determined by the individual costs associated with the specific service requested by the investigator. Investigators are also free to use the specialized equipment available in the core laboratory
Tissue Culture Core Facility
The Eppley Institute operates a tissue culture core facility that offers liquid cell culture media at substantially lower costs than commercial venders. Most common media are in stock and are available in 1 liter to 10 liter quantities. Larger quantities are available on a 2-day turn around.
Flow Cytometry Core Facility
The flow cytometry core facility is housed in the Department of Pathology and Microbiology and provides a broad range of instrumentation as well as consultation services in technical assistance and operation of instruments, data analysis and interpretation of results. The facility will sort cells for culture or for simple analysis of populations. The facility houses three state-of-the-art instruments: a Becton Dickinson FACStarPlus flow cytometer operating under Lysis II, an Ortho Cytofluorograph System 50H flow cytometer operating under Cytomation software, and a Meridian ACAS 570 Confocal Laser Scanning Cytometer.
Microarray Core Facility
The microarray core facility operates out of the Eppley Institute and provides a comprehensive service including experimental design, RNA preparation, hybridization, scanning, feature extraction, normalization and assistance with statistical analysis.
The biostatistics core is housed in the Department of Preventative and Societal Medicine and provides services on a fee for service basis. They assist with experimental design and statistical analysis.
Human Tissue Bank
The department of pathology operates a human tissue bank that includes both normal and disease tissues collected at surgery.
Protein Structure Core Facilities
- X-ray crystallography. The Eppley Institute is just establishing an X-ray facility with the latest instrumentation. This facility will be available on basically the same manner as the NMR facility as discussed above.
- Molecular Modeling. The molecular modeling facility is housed in The Eppley Institute and provides a basis for integrating computational and experimental methodologies allowing investigators to make full use of the power of modern computer technology to build, structurally optimize and optically manipulate chemical structures ranging from simple organic compounds to large proteins.
- Molecular Interaction Facility. The molecular interaction core facility operates a BIACORE 1000 instrument, which the core facility manager will train investigators to use, or will perform the experiment on protein samples provided by the investigator.
UNMC has an up-to-date animal facility that is housed in the Department of Comparative Medicine. It is responsible for animal procurement, husbandry and veterinary services.
Transgenic mouse facility The Munroe-Meyer Institute for Genetics and Rehabilitation operates a transgenic mouse facility that is available to produce knock out and transgenic mice using a fee for service mechanism.
CREIGHTON UNIVERSITY CORE FACILITIES
Microscopy Core Facility The Center for Advanced Imaging core facility is housed in the Department of Biomedical Sciences in the Criss III building. The Center has a Bio-Rad Radiance 2000 confocal microscope with a two-line 10 mW Krypton-Argon laser and a second laser, a 5 mW red diode laser (638 nm) suitable for deep red fluorophores, is also available. A monochrome video camera and video acquisition computer equipped with Image-Pro image processing software is also attached to the confocal microscope. The facility is fully networked for easy file exchange.
Flow Cytometry Core Facility
The flow cytometry core is in the Department of Medical Microbiology and Immunology and includes a Becton Dickinson FACSCalibur instrument in the Criss II building. Technical help is provided for procedures and instrumentation for investigators who wish to run the instruments themselves. Assistance is provided for interpretation and analysis of data. The facility will sort cells for culture or for simple analysis of populations.