Electron Microscopy Core Facility

MISSION

The Electron Microscopy Core Facility (EMCF) within the Department of Genetics, Cell Biology and Anatomy is located in Wittson Hall (room 2012). The EMCF provides instrumentation and technical assistance to research programs involving Transmission Electron Microscopy and Scanning Electron Microscopy. The EMCF is available to all University of Nebraska personnel, as well as outside institutions and industrial users. A full time technologist and a facility supervisory committee are available for consultation. The facility is supported, in part, by user fees to cover supplies, instrument time and the technologist's time to carry out any part of the project. A fee schedule is available on request.

EQUIPMENT

The EMCF installed a new FEI Tecnai G2 Spirit transmission electron microscope (2009). The instrument has a goniometer stage for precise tilting of specimens for stereoscopic images or to orient specimens for optimum viewing. Images are acquired digitally with an AMT digital imaging system.Electron Microscopy Core Facility equipment

For scanning electron microscopy the facility is equipped with a FEI Quanta 200 SEM (2006). This instrument can operate in conventional high vacuum mode, variable pressure low vacuum and environmental SEM modes. This range of modes allows for examining non-conductive, beam sensitive and wet specimens as well as conventionally prepared SEM samples. The Quanta 200 is also equipped with a new Bruker AXS Quantax XFlash 4010 x-ray microanalysis detector, allowing for elemental microanalysis of SEM samples. All images are acquired digitally.

Electron Microscopy Core Facility equipment

The facility has supporting equipment for all standard biological specimen preparation protocols. For TEM this includes a Leica Ultracut ultramicrotome, LKB glass knifemakers, diamond knives, centrifuges, embedding oven, a Pelco UV-2 cryochamber for low temperature embedding for immunocytochemistry and a Pelco Biowave variable wattage microwave fixation system.Electron Microscopy Core Facility equipment

Support equipment for SEM includes a Hummer VI sputter coater, a Pelco critical point drying apparatus, and a Denton vacuum evaporator.

TECHNIQUES

Technical expertise is available in the areas of Transmission Electron Microscopy, Scanning Electron Microscopy, Light Microscopy, Ultramicrotomy, Specimen Preparation, Immunocytochemistry, Negative Staining, Metal Coating, Digital Imaging and Stereology.

TOURS

Any interested individuals or groups may schedule a tour of the Core Electron Microscopy Research Facility. Please contact Public Affairs for more information and to schedule tours.

EDUCATION

There are two related graduate level courses in electron microscopy. The introductory techniques course is Fundamentals of Electron Microscopy, CBA No. 930, which is taught in the spring semester. An advanced independent study course, Selected Topics in Electron Microscopy, CBA No. 931 is taught at varying times.

Contact Information
Tom Bargar, M.S., MSA Cert.
Electron Microscopy Specialist
Location: WHM Room 2012
Phone: 402-559-7347
E-mail:tbargar@unmc.edu

Tom Bargar
Tom Bargar
Electron Microscopy Specialist


Andrew T. Dudley, Ph. D.
Core Director
Location: 6064 DRC II
Phone: 402-559-2820
Email: andrew.dudley@unmc.edu

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