James Eudy, Ph.D.

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James Eudy, Ph.D. Assistant Professor Director of DNA Microarray Core Facility University of Nebraska Medical Center (Biochemistry and Molecular Biology) 1995 Phone: (402) 559-3940 Fax: (402) 559-4001 Email: jdeudy@unmc.edu
Teaching Cell and Molecular Processes Core:Yearly lectures to first year medical students involving several areas of human genetics and molecular biology including gene mapping, gene therapy, and functional genomics. Biochemistry 850: I present a lecture in this laboratory course offered by the Department of Biochemistry at UNMC. This lecture covers the history, laboratory techniques, and fundamental research applications of DNA Microarray Technology.
Research – Interests Molecular Genetics of Hearing and Vision; Micronutrient Transporters and Birth Defects; Functional Genomics A primary interest and current responsibility is directing the DNA Microarray Core Facility. The facility is comprehensive in scope and provides microarray service and support for both the spotted DNA array and the Affymetrix GeneChip System platforms. The laboratory routinely processes many of the expression and genotyping arrays offered by Affymetrix. The facility is fully equipped with all the necessary instrumentation for printing up to 10,000 genes for human, rat, and mouse, as well as, hybridizing, scanning and analyzing spotted arrays. Collectively, the Affymetrix and spotted array platforms allow researchers to measure the activity of tens of thousands of genes in parallel in a variety of model organisms.
Publications listed in PubMed Research Publications Ruthermund CA, Gopalakrishnan VK, Eudy JD, and Vishwanatha JK. Casodex treatment induces Hypoxia-related gene expression in the LNcAP prostate cancer progression model. BMC Urology 5(1) 5, 2005. Shen, Y, Iqbal J, Xiao, L, Lynch, RC, Rosenqald, A, Staudt LM, Sherman, S, Dybkaer, K, Zhou, G, Eudy JD, Delabie, J, McKeithan TW, Chang WC. Distinct gene expression profiles in different B-cell compartments in human peripheral lymphoid organs. BMC Immunology 5-20, 2004. Wiedmann S, Eudy JD, Zempleni J. Biotin supplementation causes increased expression of genes encoding interferon-γ, interleukin-1β, and 3-methylcrotonyl-CoA carboxylase, and causes decreased expression of the gene encoding interleukin-4 in human peripheral blood mononuclear cells. Journal of Nutrition 133:716-9, 2003. Huang DH, Eudy JD, Uzvolgyi E, Davis JR, Talmadge CB, Pretto D, Weston MD, Lehman JE, Zhou M, Seemayer TA, Ahmad I, Kimberling WJ, Sumegi J. Identification of the mouse and rat orthologs of the gene mutated in Usher syndrome type IIA and the cellular source of USH2A mRNA in retina: a target tissue of the disease. Genomics 80:195-203, 2002. Finnell RH, Gelineau-van Waes J, Eudy JD, Rosenquist TH. Molecular Basis of Environmentally-Induced Birth Defects. Annual Review of Pharmacology and Toxicology. 42:181-208, 2002. Weston MD, Eudy JD, Fujita S, Yao SF, Sumegi J, Kimberling WJ. Genomic structure and identification of novel mutations in usherin, the gene responsible for usher syndrome type IIa. American Journal of Human Genetics. First two authors contributed equally to this manuscript. 66:1199-1210, 2000. Spiegelstein O, Eudy JD, and Finnell, RH. Identification of two novel folate receptor genes in mouse and humans. Gene. First two authors contributed equally to this manuscript. 27;258:117-25, 2000. Eudy JD, Spiegelstein O, Barber RC, Wlodarczyk BJ, Talbot J, Finnell, RH. Identification and characterization of the human and mouse SLC19A3 gene: a novel member of the reduced folate family of micronutrient transporter genes. Molecular Genetics and Metabolism. First two authors contributed equally to this manuscript. 71:581-590, 2000. Eudy JD, Sumegi J. Cellular and molecular aspects of Usher Syndrome. Cellular and Molecular Life Sciences 56:258-267, 1999. Eudy JD, Yao SF, Ma-Edmonds M, Talmadge C, Weston MD, Kimberling W, Sumegi J. Isolation of a gene encoding a novel member of the orphan hormone receptor superfamily from the Usher Syndrome Type 2a locus on Chr 1q41. Genomics 50:382-384, 1998. Eudy JD, Weston MD, Yao SF, Hoover DM, Ma-Edmonds M, Yan D, Cheng JJ, Beisel K, Ayuso C, Cremers C, Davenport S, Moller C, Talmadge C, Tamayo M, Swaroop A, Kimberling WJ, Sumegi J. A novel gene encoding a protein with extracellular matrix motifs is mutated in Usher syndrome type 2a. Science 280:1753-1757, 1998.