The Biosafety Manual has been developed by the Office of Regulatory Affairs and the Institutional Biosafety Committee (IBC) at the University of Nebraska Medical Center (UNMC). The manual is part of the UNMC / University of Nebraska at Omaha (UNO) and Nebraska Health Center Biosafety Program instituted to accomplish the following goals:
- to protect personnel from exposure to infectious agents,
- to prevent environmental contamination,
- to provide an environment for high quality research while maintaining a safe work place,
- to comply with applicable federal, state and local requirements, and
- to create a secure laboratory environment to prevent unauthorized utilization of the biological agent.
The Biosafety Manual provides university-wide safety guidelines, policies and procedures for the use and manipulation of biohazards. Although the implementation of these procedures is the responsibility of the Principal Investigator (PI), its success depends largely on the combined efforts of laboratory supervisors and employees. Planning for and implementation of biological safety must be part of every laboratory activity in which biohazardous materials are used.
Recommendations in the manual define a "standard of practice" that laboratories should follow.
In general, the handling and manipulation of biological agents and toxins, including recombinant DNA molecules, requires the use of various precautionary measures depending on the material(s) involved. This manual will provide assistance in the evaluation, containment and control of these biohazards. It is required that all parties involved or working with these materials be familiar with the contents of this manual, complete the required training, and that they seek additional advice when necessary. The IBC Chairperson, as well as the Biosafety Officer (BSO), are available at UNMC to assist in this endeavor.
- Biosafety Program Administration
- Biological Safety and Biosafety Levels
- Classification of Infectious Agents on the Basis of Hazards
- Biohazardous Materials
- Human Gene Transfer Experiments
- Possession, Use, and Transfer of Select Agents and Toxins
- Rules, Regulations, and Biosafety Guidelines
- Routes of Infection
- Administration Controls
- Engineering Controls
- Personal Protective Equipment
- Recommended Work Practices
- Biological Risk Assessment
- Working with Tissue Cultures
- Prevent Transmission of Tuberculosis
- Clinical Laboratory Guidelines
- Use of Animals in Research
- Transportation of Biological Materials
- Biohazardous Waste
- Blood Borne Pathogens Program and Exposure Control Plan
- Hand Hygiene in Research Laboratories
- Appendix A Select Agents
- Appendix B USA PATRIOT Act
- Appendix C Properties and Applications of Disinfectants