Imaging Core

The Integrated Biomedical Imaging Facility at Creighton University is a new multi-user core that is closely related to the scientific focus in structural biology. Core technology includes the LSM 510 METANLO confocal scanning system with three lasers (Argon 458/477/488/514 nm, Green HeNe 543 nm, Red HeNe 633 nm. The confocal is built on a Zeiss AxioPlan 2IE MOT motorized upright microscope, with the following objectives: Plan Neo 40x oil 1.3 numerical aperture (n.a.) with differential interference contrast (DIC); Plan 63x 1.4 n.a. DIC; Achroplan 40x 0.8 n.a. water DIC, for live cell work; C-Apochromat 40x 1.2 n.a. DIC ,for fluorescence correlation spectroscopy; Plan Apo 10x; Plan Neo 20x 0.75 n.a. DIC. Initial funding for the core was through an National Science Foundation EPSCoR grant. The Core can image with most fluorophores, in either single-photon or multi-photon modes, with resolution approaching the theoretical limit (222 nm) The META multi-channel system enables separation of fluorophores with closely-spaced emission peaks by linear un-mixing. The types of experiments available far surpass those of conventional high resolution confocal imaging. Particular emphasis is placed on fluorescence-based and other dynamic imaging modalities, including:

  • Spectral Imaging using the META detector, including linear un-mixing for separation of fluorophores with overlapping emission bands.
  • Fluorescence Correlation Spectroscopy (FCS) for the study of the dynamics of molecular interactions.
  • Fluorescence Recovery After Photobleaching (FRAP), for the study of molecular mobility.
  • Fluorescence Resonance Energy Transfer (FRET), for the study of molecular interactions.
  • Fluorescence Lifetime Imaging (FLIM), an enhanced approach to FRET.
  • Total Internal Reflection Fluorescence (TIRF), for the study of single molecule dynamics.
  • Complex time-series experiments from seconds to days.
  • Imaging of ionic currents of pH.
  • Live cell imaging in culture with temperature control.
  • Metabolic imaging.
  • Deep brain or deep tissue imaging in vitro or in vivo.

core director
Dr. Richard Hallworth
Director CUIBIF Integrated Biomedical Imaging Facility

Department of Biomedical Sciences
Creighton University
2500 California Plaza
Omaha, NE 68178

Office: 402-280-3057
Fax: 402-280-2690
hallw@creighton.edu

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