- To establish an integrated proteomics program to support researchers at the UNMC in their efforts to seek extramural funding.
- To provide high quality services.
- To facilitate collaborative scientific effort with other institutions of higher learning in the state of Nebraska.
- To provide a state-of-the-art environment for education of researchers in techniques of mass spectrometry and proteomics.
The Protein Structure Core located in the Department of Biomedical Sciences at Creighton University Medical Center helps investigators to understand structural aspects of biological activities of proteins. The genome sequencing projects are providing vast quantities of new information that is invaluable for identifying and characterizing gene products. However, primary structure is only part of the complete structural characterization required for understanding mechanisms of protein functions. The three-dimensional (3D) structures must also be known. For initial structure-function studies, fragments/domains of proteins can be efficiently synthesized using solid phase peptide synthesis. These fragments could be structural, functional domains or epitopes for raising antibodies. The selection of fragments/domains is accomplished by using structural bioinformatics tools. 3D structural characterizations of proteins, protein fragments and synthetic polypeptides are performed in solution using chiroptic spectroscopy (ECD and VCD). These methodologies, although at low resolution, provide the initial structural information and are important in using other high resolution techniques for determining the finer structure. Structures obtained with ECD and VCD spectroscopy are further characterized with molecular dynamics (MD) simulations to reveal fine structural details and structural stabilities. Furthermore, homology modeling and molecular docking complemented with MD simulations is used in the design of functional analogs of interacting domains of polypeptides and proteins.
Director Mass Spectrometry and Proteomics Core Facility
University of Nebraska Medical Center
985800 University of Nebraska Medical Center
Omaha, NE 68198-5800
Dr. Sandor Lovas
Department of Biomedical Sciences
2500 California Plaza