Growth of cells in vitro to produce immunoglobulin-containing supernatant.
Small scale in vitro production of antibody. Supernatants are saved as cells are archived, or cells are grown to extinction to boost antibody concentration. It is common for a hybridoma cell line to produce 5-20 µg of antibody per ml of supernatant - 100 ml could yield up to 2 mg.
Immunoglobulin
Weeks
COST
1-5 mg
1-2 weeks
$60
5-25mg
2-3 weeks
$120
25-50 mg
3- 4 weeks
$260
50-75 mg
4-5 weeks
$350
75-100 mg
~8 weeks
$550
Large scale in vitro production of antibody. The Integra CL 1000 System is used for large scale antibody production. Concentrations of antibody can reach 1 mg/ml. The specialized flask allows increased cell and antibody concentrations while decreasing the amout of serum required for culture.