Purification of immunoglobulin from supernatant or sera, using FPLC & AKTA instrumentation
Protein A
$65.00/200 ml supernatant (approximate yield 2 mg)
Protein G
$60.00/200 ml supernatant (approximate yield 2 mg)
murine IgM
$80.00/200 ml supernatant (approximate yield 2 mg)
Affinity Chromatography
antigen will be coupled to a 1 ml or 5 ml HiTrap resin/blocked and ready for antibody purification
~$80 for a 1 ml column ~$225 for a 5 ml column
Anti - bovine IgG
$90.00/200 ml supernatant
Chelating Chromatography
for His tag purification - $200/100 ml supernatant
Glutathione Affinity Chromatography
for GST tag purification - $250/100 ml supernatant
The costs listed are to be used to estimate the price of services performed in the Monoclonal Antibody Facility for UNMC faculty. Non-UNMC academic users are charged an institutional overhead fee of 25% (labor costs for academic users are supported by the Nebraska Research Initiative). Corporate product development can be contracted.