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DNA Sequencing

DNA Synthesis

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Online Synthesis Request Form

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DNA Sequencing is performed using a Perkin-Elmer Applied Biosystems Model 377 DNA Sequencer.  This instrument can analyze up to 96 samples at a time and resolve 400-1000 base pairs.  Using dye terminator cycle sequencing chemistry, direct sequencing of  DNA from plasmids, phage or PCR products is possible using universal or custom primers.  Please inquire about 10K or larger template sequencing.  Investigators may choose to analyze their own raw data (General Analysis) or have the MBCL interpret the data and generate finished results (Custom analysis).  The turnaround time is typically 1 week or less.  Alignments of multiple sequences are possible using Sequence Navigator software.

Genewalking services are offered, utilizing both the DNA Synthesis and Sequencing capabilities of the laboratory.  Generally, a plasmid containing an insert of interest is submitted for analysis and sequence is obtained from a vector-based primer.  From the generated sequence, an optimized primer is designed, synthesized and another round of sequencing is performed.  In this way, several kilobases of DNA sequence data can be obtained in a short amount of time, adding cycles of sequencing and synthesis as needed.

DNA Fragment Analysis (Genescan) is also performed on the Model 377 providing 1-2 bp resolution of  DNA fragments ranging from 75 to 1200 base pairs in size.

Template and Primer Information:

• Factors which affect Sequence Quality
• General Rules for Primer Design
• Primers provided by the Core Lab

Forms and Submitting Samples:

• How to fill out a DNA Request Form
• DNA Request Form

Sequencing Method

• Methodology for DNA sequencing for use in publications

Download Software 

•  Software