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DNA Sequencing

DNA Synthesis

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Online Synthesis Request Form

Current Prices

1. For 8 or more samples with the same primer ask the Core Lab for a multiplesample sheet form (new prices). Otherwise, use one request sheet per primer.

2. Complete all requested information at the top part of the form. Account #’s are required before any work will be done. For bookkeeping purposes each form must be completed in its entirety.

3. Place an X in the box for the Double Stranded or PCR product and fill out corresponding blank lines with requested information.

A. We are now requiring labs to measure their own Double Stranded plasmid samples using our fluorometer. The fluorometer is setup in Room 6001 and the protocol is located there.

B. We cannot measure PCR products via fluorometry so a gel picture of the POST-PURIFIED PCR product is required.  Inciate the sample volume loaded, a size marker and amount loaded.  Tape the gel picture to the front part of the sheet.  

4. Place an x in the box for Primer Selection. Remember ONE PRIMER PER SHEET!  Standard Primers are listed below:  Please fill in the primer selection

M13-21For            T3 Glprimer1             T7 terminator                   PGEX3'

M13Reverse          T7 Rvprimer3           T7eevpromotor               PGEX5'

M13-40For             pucM13                     Polyhedrin For                KS

SP6                        pucM13Rev               Polyhedrin Rev               SK

For Custom primers limit name to 5 characters and provide at 1uM NO EXCEPTIONS!   Also provide the Tm.

5. Select Analysis Type at bottom. There are discounts for 8 or more samples with      the same primer. See Core Lab for details and special forms.

6. We can help you set up your MAC computer for file sharing. PLEASE SEE US!

We will not transfer text data via email. Please set up a molbio account at:

http://molbio.unmc.edu

Note:Because of potential Virus problems we will no longer transfer data via your floppy disks. We will provide virgin disks for a fee.

HOW TO LABEL YOUR TUBES:Please provide DNA samples in 1.5 ml tubes and primers in 0.5ml tubes. The Core Lab will provide them if necessary.

Sample name should match EXACTLY with the name on the sample sheet. Please use a BLACK SARSTEDT pen (available from Eppley stockroom Material # 103401) on the TOP of the tube. Do not label the sides-this rubs off.