REQUEST FOR DNA SEQUENCING

REQUEST FOR DNA SEQUENCING
MBCL, Room 6004, Eppley Institute, Ph. 559-6075/Fax 559-4651/Campus Zip 6805

Principal Investigator: __________________                                Date:__________
             Department:__________________                    Sample Name:_____________(7 character limit)
               Account #: ______________             Purification Method:_____________
Contact Person/ph #:__________________                      Concentration:__________ng/ul (via fluorometry)

COMPLETE SECTIONS I, II, & III BELOW. USE ONE SHEET PER PRIMER/TEMPLATE PAIR
Submit All Samples in Water/Buffer WITHOUT EDTA

I
SAMPLE TYPE: CHECK ONE OF THE FOLLOWING BOXES
Double Stranded Vector Name_____________Vector Size_____bp Insert Size_____bp Total Construct_____bp
Single Stranded Vector Name____________________
Is sample G-C rich? Yes___ No___ DMSO for sample?____ Sample returned?_______ (Sample held for 30 days)

ATTACH A GEL PICTURE OF PURIFIED PCR SAMPLE HERE.
Indicate volume loaded
Include size marker/# ug loaded

PCR rxn annealing temp________(REQUIRED)
Length of insert or PCR product_______bp
Is sample G-C rich? _____
DMSO for sample?____
Sample returned?______

II
PRIMER TYPE: CHECK ONE OF THE FOLLOWING BOXES
STANDARD PRIMERS (PROVIDED): CIRCLE ONE SELECTION
(use additional sheets as needed)
M13-21 Forward           T3                    puc M13 For            KS                           T7 terminator
M13 Reverse                T7                puc M13 Rev           SK                            T7 eev promotor
M13-40 Forward           PGEX5'          GL primer 1               Polyhedrin For         BGHrev
SP6                               PGEX3'          RV primer 3              Polyhedrin Rev

CUSTOM PRIMERS: PROVIDE AT 1uM                                    NEW PRIMER (in synthesis)
Primer Name:____________(limit of 5 character)                         Primer Name________________
Tm of primer_____________                                                      Core Number______________
( Provide a MINIMUM of 10 ul per reaction)                                  Ext. Coefficient___________

III
ANALYSIS/FEES (make selection):
______ General analysis includes: raw data-must be edited by user via computer $20 per sample
______ Custom analysis includes: Editing, Color Electropherogram, Sequence Printouts $30 per sample $35 for non-university customers
______ Sequence Alignments:$5.00 /2 primers (More than 2 primers, inquire for pricing)
______ Placement into Folder for GCG work, FREE!! Login:____________________
A $7.00 setup fee is charged to cover the cost of chemicals for samples that do not provide data.
A $10.00 setup fee is charged for a sample that is setup twice without results.

DO NOT WRITE BELOW THIS LINE. FOR CORE USE ONLY
Core #___________           Alignment Charge:$________
Gel #:_________                              Charge:$________                   Total Charges: $_______
Gel #:_________                              Charge:$________         Month/Year Charged:___________