DNA Sequencing

DNA Synthesis

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Current Prices

1.  DNA template quality and amount
        a.  The conditions under which the culture is grown
        b.  The host strain selected.
        c.  Mixed templates

2.  Contamination in the DNA sample
        a.RNA, Salt, Ethanol, Phenol, Chloroform

3.  Primer Quality
        a.  Significant amounts of n-1 primers and short derivatives can cause problems
        b.  GC composition of primer and annealing temperature 

4.  Effective hybridization of primer and DNA
        a.  Tm of primer
        b.  Design of primer

5.  Actual sequence of DNA template
        a.  GC-rich templates
        b.  Homopolymeric regions

6.  Size of  Template
        a.  BACS