The Shared Resource provides services for all high-throughput
screening applications to
Resuspend, distribute, and store siRNA screening sets into 96- and 384-well formats.
Provide positive and negative universal control siRNAs.
Perform HTS processes of cell plating and transfection.
Perform HTS steps of endpoint and readout assays.
Provide protocols and assistance in experimental design, statistical analysis, and data management.
Maintain all instrumentation and assist in ordering materials and reagents.
Provide assistance with validation studies.
Provide special pricing on screening and validation reagents (e.g. siRNA, transfection reagents, and controls)
Provide transfection reagents and assay-specific siRNA controls.
Provide cell lines, reagents, culture medium, plasticware, and disposables.
Provide read-out assay reagents.
Perform validation studies.
The Shared Resource will recoup costs associated with the purchase of the siRNA library, set-up plasticware and reagents, labor, and service contracts on instrumentation via charge-back mechanism (~$5,700-$6,100/screen). All other costs will be through direct purchase by each principal investigator.
High-Content Imaging and Analysis.
the future the Shared Resource anticipates offering