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DNA Sequencing

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The following standard primers are provided by the Core Lab for in house DNA Sequencing purposes.  These primers are also for sale in 1 O.D. aliquots.  

                Primer Name                  Sequence 5'-3

                    T7                                TAA TAC GAC TCA CTA TAG GG 

                 T7 terminator                GCT AGT TAT TGC TCA GCG G     

                 T7 eev promotor            AAG GCT AGA GTA CTT AAT ACG A 

                 T3                                AAT TAA CCC TCA CTA AAG GG

                 Sp6                               ATT TAG GTG ACA CTA TAG 

                     M13-21 For                   TGT AAA ACG ACG GCC AGT 

                     M13 Rev                       CAG GAA ACA GCT ATG ACC

                     M13-40 For                   GTT TTC CCA GTC ACG AC

                 PGEX5'                        GGG CTG GCA AGC CAC GTT TGG TG

                 PGEX3'                        CCG GGA GCT GCA TGT GTC AGA GG

                 BGH Rev                      TAG AAG GCA CAG TCG AGG

                 Polyhedrin For               AAA TGA TAA CCA TCT CGC

                     Polyhedrin Rev              GTC CAA GTT TCC CTG

                     GL primer 1                   TGT ATC TTA TGG TAC TGT AAC TG

                 RV primer 3                   CTA GCA AAA TAG GCT GTC CC

                  KS                                TCG AGG TCG ACG GTA TC

                 SK                                 CGC TCT AGA ACT AGT GGA TC