RNA Expression: The core facility has the complete Affymetrix GeneChip system and can process RNA samples for a variety of organisms. This includes chips for all model organisms for which Affymetrix has a chip available including human, mouse, rat, drosophila, c. elegans, yeast and arabidopsis.
Genome-wide SNP Analysis: In addition to expression analysis the Affymetrix system can be used to perform genome-wide SNP analysis. These arrays can also be used to determine genomic copy number alterations.
Spotted Microarray and mirVana miRNA
Labeling and Hybridization: The core facility will label the RNA samples and perform the hybridizations to the slides for investigators.
Slides Available: 30k Human, 30k Mouse, 10k Human, 10k Mouse, 10k Rat and miRNA arrays
New! Genome-Wide spotted oligo microarrays for human and mouse experiments. The core facility can now offer you genome-wide expression profiling for your experiments involving human or mouse RNA samples at a reduced cost. The facility can compare the expression profile of ~ 30,000 genes between two samples in either mouse or human for a total cost of approximately $250.00. The slides used are from the company known as Phalanx Biotec.
Custom Array Printing: The core facility employs the use of a OmniGrid 100 spotting robot and will print custom oligo and cDNA arrays for users.
Scanning: The facility employs the use of an Axon 4000B Laser Scanner.
Image Analysis: GenePix is the image analysis software utilized for spotted microarray images.
Agilent Bioanalyzer - RNA Integrity Analysis
In our facility we employ the use of an Agilent Bioanalyzer which will assess the purity, potential degradation and genomic DNA contamination of RNA.
Analysis
Statistical Analysis: The Microarray Core Facility can assist with initial analysis to determine fold-changes in genes. For in-depth statistical analysis the user is referred to statisticians and computer scientists at UNMC that routinely provide those services.
Pathway Analysis: The Microarray Core Facility has purchased access to the analysis program known as Ingenuity Systems.Users are encouraged to utilize this program to further analyze their microarray data.