Molecular Interaction Core Facility

Molecular Interaction Core Facility

The BIACORE is an optical biosensor that uses surface plasmon resonance (SPR) for real-time monitoring of macromolecular interactions without the need for labels.  Applications include affinity measurements, binding kinetics, active concentrations, binding specificity and epitope mapping. The technology is for use in drug discovery, antibody screening, ligand fishing and therapeutics. The BIACORE 3000 samples include small molecules, crude extracts, lipid vesicles, DNA, RNA, viruses, bacteria or eukaryotic cells. Technological advantages include minimal sample consumption, recovery of surface bound samples for MS analysis, multi-sample analysis and measurement of weak binding events.

The Molecular Interaction Core Facility at UNMC, currently has a BIACORE 3000 and a BIACORE 1000 upgrade using BIAcontrol software, as well as BIAsimulation software  and BIAevaluation software.  The facility provides access to these instruments at a nominal cost for investigators who wish to use this technology in their research. In addition, the core facility provides technical assistance and training for using the BIACORE and expertise in analysis of kinetic data when needed.

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6-2006