DNA Requirements and Isolation
We require 100-200ng DNA quantitated by Picogreen or Nanodrop and dried down in 96-well plates. Per 96-well plate, we recommend duplicating 5 samples and including two empty wells for positive and negative control samples. DNA must be eluted in water as TE may interfere with the genotyping assay. A260:280 of 1.7-2.0 is required for Sequenom genotyping unless the user agrees to assay these samples at their own risk. Users are responsible for providing documented, high-quality DNA for genotyping assays. Investigators can retain the labs services to quantitate the DNA after which time only samples meeting stringent QC will be genotyped.
| Sample Type | Isolation Kit |
| Blood (200 ul) | Qiagen QiaAMP mini #51304 |
| Blood (2 ml) | Qiagen QiaAMP midi #51183 |
| Blood (2-10 ml) | Qiagen QiaAMP maxi #51192 |
| Buccal Swab | Contact mkaplan@email.arizona.edu Kit and Automated Isolation |
UNMC BioBanks
http://ctsa.unmc.edu/template_view.cfm?PageID=75
Commercial Samples
http://www.capitalbiosciences.com/fresh-tissue
http://www.concomatrix.com/
http://www.iiam.org/humanTissueForResearch.php
