DNA Requirements and Isolation
We require 100-200ng DNA quantitated by Picogreen or Nanodrop and dried down in 96-well plates. Per 96-well plate, we recommend duplicating 5 samples and including two empty wells for positive and negative control samples. DNA must be eluted in water as TE may interfere with the genotyping assay. A260:280 of 1.7-2.0 is required for Sequenom genotyping unless the user agrees to assay these samples at their own risk. Users are responsible for providing documented, high-quality DNA for genotyping assays. Investigators can retain the labs services to quantitate the DNA after which time only samples meeting stringent QC will be genotyped.
|Sample Type||Isolation Kit|
|Blood (200 ul)||Qiagen QiaAMP mini #51304|
|Blood (2 ml)||Qiagen QiaAMP midi #51183|
|Blood (2-10 ml)||Qiagen QiaAMP maxi #51192|
|Buccal Swab||Contact firstname.lastname@example.org
Kit and Automated Isolation