Instrumentation & Chemistry

Instrumentation

Sequenom MassArray: The principle of the iPLEX system is the extension of an oligonucleotide probe over the SNP site in a PCR product with dideoxynucleotides, to produce different sized products for each allele of a SNP. These extension products are then analyzed by Matrix-Associated Laser Desorption Ionization Time of Flight (MALDI-TOF) mass spectrometry, permitting precise determination of their size, which is converted into genotype information.

Amplification, Primer Extension, Detection

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