Services and Prices

MSPCF Pricing per Sample

Prices subject to change

(Industrial accounts, please contact us for prices)

      
Mass Spectrometry Service Instrument UNMC Affiliates Other Academic Institutions
Protein Identification 5600 TripleTOF $75 $115
  LTQ Orbitrap $50 $75
  LTQ Velos $50 $75
  4800 MALDI $50 $75
       
 iTRAQ (4 samples, 4-plex)1 5600 TripleTOF $3200 $4800
 iTRAQ (8 samples, 8-plex)1 5600 TripleTOF $3200 $4800
Determination of Molecular Weight:      
  • Proteins and Peptides (under 25K)
 4800 MALDI $30 $45
  • Others needing usual matrices
 4800 MALDI $55 $85
Sample Preparation      
 In-gel tryptic digest (includes zip-tip clean-up)   $25/band  $40/band
 In-solution tryptic digest (includes zip-tip clean-up)   $30/sample  $45/sample
 ZipTip - 1 sample   $9/sample  $15/sample
  •  each additional sample
   $3/sample  $5/sample
 Sample clean-up (PepClean C18)-1 sample   $80/sample  $120/sample
  •  each additional sample (up to 12 total)
   $10/sample  $15/sample

Sample clean-up (MCX)-1 sample

   $25/sample  $40/sample
  • each additional sample (up to 12 total)
   $8/sample  $15/sample

 Sample concentration (centrifugal filter) less than 2mL - 1 sample

  

 $40/sample

  $60/sample
  •  each additional sample (up to 8 total)
  

 $10/sample

  $15/sample

Sample concentration (centrifugal filter) 2-15mL - 1 sample

  

 $85/sample

  $130/sample
  •  each additional sample (up to 8 total)
  

 $20/sample

  $30/sample

Phosphopeptide enrichment (includes phosphopeptide spin tip and graphite cleanup -- Does not include C18 pre-cleanup) - 1 sample

  

 $170/sample

  $255/sample
  •  each additional sample (up to 12 total)
  

 $25/sample

  $40/sample
Sample Purification through HPLC Shimadzu HPLC $50/mg  $75/mg
Depletion of albumin and IgG (Sigma ProteoPrep spin columns, 25-50ul)   $100/sample  $150/sample
  • each additional sample (up to 10 total)
   $55/sample  $85/sample
Sample Fractionation      
 1D gel electrophoresis standard 4-12% Bis-Tris (up to 11 samples)   $60/gel  $90/gel
Coomassie staining   $25/gel  $40/gel
Sypro ruby staining (includes imaging)   $45/gel  $70/gel
Offgel - 12 well (includes MCX and PepClean cleanup)   $500  $750
  • each additional Offgel (2nd and 3rd)
   $200 ea  $300/ea

Western blot (investigator provides all antibodies)

      

Includes cost of standard 1D, membrane transfer, incubation and development of 1 antibody

   $165  $250
  •  each additional antibody on same membrane
   $45  $70
Consultation   $150/hour $175/hour
Training   $80/hour Not Available
Other Projects   $60/hour $120/hour

1Data from iTRAQ experiments will be processed using ProteinPilot 4.0 and exported into 2 Excel files containing information on protein/peptide identification, confidence, quantitation, statistical analysis (as provided by Protein Pilot) and spectra information.