Recommended Sample Amounts for Mass Spectrometric Analysis*
| Type of Service | Instrument | Minimum Amount | Optimal Amount** | Notes |
| Protein Identification | 5600 TripleTOF | 15 fmol | 500 fmol | Coomassie Blue stained gel bands or in solution. |
| LTQ/Velos | 15 fmol | 500 fmol | ||
| 4800 MALDI | 100 fmol | 500 fmol | ||
| Protein Mass | 4800 MALDI | 100 fmol | 1 pmol | Concentrations above 1 pmol/µL in volatile solvents free of non-volatile buffers, detergents, salts, urea, glycerol, etc. Suitable solvents are water, acetonitrile/water or methanol/water mixtures or dilute, volatile buffers (e.g., 1 mM NH4HCO3). |
| Peptide Mass | 4800 MALDI | 100 fmol | 1 pmol |
Concentrations above 1 pmol/µL in volatile solvents free of non-volatile buffers, detergents, salts, urea, glycerol, etc. Suitable solvents are water, acetonitrile/water or methanol/water mixtures or dilute, volatile buffers (e.g., 1 mM NH4HCO3). |
*After tryptic digest and sample processing, expected recovery is 45-50%, but can be as low as 30%. This must be taken into account when submitting samples.
**Based on well ionized peptides
