Histocompatibility

The Histocompatibility Laboratory, under the lead of James Wisecarver, M.D., Ph.D., provides testing services to support solid organ and bone marrow transplant programs at The Nebraska Medical Center (TNMC) and throughout the region. In addition, the laboratory provides state-of-art human leukocyte antigen (HLA) typing to assist in assessment of disease associations and in a variety of medical situations, including platelet transfusion and drug sensitivity profiling.

A broad menu of HLA typing, HLA antibody screening, and lymphocyte crossmatching services are offered including:

  • HLA-A, B, C, DR and DQ loci typing, including both serological and molecular methods, ranging in resolution from low (antigen-level) to high (allele-level).
  • HLA antibody identification and monitoring by solid phase Luminex methodology for antibodies to HLA-A, B, C, DR and DQ.
  • Lymphocyte crossmatch by the complement-dependent cytotoxicity (CDC) method as well as by flow cytometric methods.

The laboratory is accredited by the American Society for Histocompatibility and Immunogenetics (ASHI). Laboratory technologists are certified by the American Society of Clinical Pathology (ASCP), including five who have earned the “Histocompatibility Specialist” designation (CHS) from American Board of Histocompatibility and Immunogenetics (ABHI).

The laboratory directors and staff are available for consultation. General questions regarding testing availability can be directed to Sophie Hauxwell 402 559-5104 or to the laboratory 402 559-7625. Medical or technical questions can be directed to either Catherine Gebhart, Ph.D., or Dr. Wisecarver 402 559-7625.

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