Molecular Diagnostics

The Molecular Diagnostics laboratory is a full-service clinical laboratory offering tests in all areas of molecular diagnostics, including infectious disease, inherited conditions, molecular hematology, molecular oncology, and pharmacogenetics. The comprehensive test menu is continually being updated with the newest diagnostic tests to help clinicians and pathologists provide the most appropriate and state-of-the-art care. Multiple full time faculty members are involved with clinical case reporting and test validation. Timothy Greiner, M.D., the laboratory’s medical director, is an expert in molecular hematopathology and Cathy Gebhart, Ph.D., the technical director, has expertise in molecular infectious disease. Julia Bridge, M.D., subspecialty boarded in Clinical Cytogenetics, is an expert in molecular sarcoma pathology as well as other solid tumors. Allison Cushman-Vokoun, M.D., Ph.D., is board certified in Molecular Genetic Pathology and has expertise in solid tumors and inherited diseases.

For medical or technical questions regarding the Molecular Diagnostics laboratory, please contact Dr. Greiner 402-559-4186.

HLA Serology 402-559-7630
Solid Tumor 402-559-7745

Available Assays:
HLA - A & B Typing
HLA - DR/DQ Typing
Lymphocyte Crossmatch:
  1. Recipient
  2. Donor
Auto Lymphocyte Crossmatch
T and B cell Flow Cytometry Crossmatch
Cytotoxic Antibody Screen

Ewing's Sarcoma, t (11;22)
Rhabdomyosarcoma, t (2;13)
Synovial Sarcoma, t (X;18)
KRAS mutation detection (codon 12, 13 and 61)
BRAF mutation detection c.1799T>A (p.V600E)
Microsatellite Instability (MSI) Analysis

HLA Molecular 402-559-7630 Hematopathology 402-559-8556

Available Assays:
HLA A, B, C Typing by SSOP, SSP, or SBT (Sequence Based Typing)
HLA DR/DQ Typing by SSOP or SSP or SBT (Sequence Based Typing)
Disease Associations:
B27; Narcolepsy (DQ); Others, inquire

Available Assays:
B-cell Clonality
Bc1-1, t(11;14)
Bc1-2, t(14;18)
Bcr/Abl, t(9;22) (p190 and p210)
Bcr/Abl, t(9;22) quant (p210)
JAK2, V617F Mutation Assay
FLT3 3 ITD Mutation Detection
T-cell Clonality

DNA Identity 402-559-7220 Infectious Disease 402-559-7744
Available Assays:
Parentage Testing
Bone Marrow Engraftment
Tissue Block Identification

Available Assays:
Adenovirus DNA Detection
BK Virus DNA Detection and Quantitation
Bordetella Pertussis DNA Detection
Cytomegalovirus (CMV) DNA Detection & Quantification
Enterovirus RNA Detection
Epstein-Barr Virus (EBV) DNA Detection & Quantitation
Hepatitis B Virus (HBV) Quantitation
Hepatitis C Virus (HCV) Quantitation
Hepatitis C Virus (HCV) Genotyping
Herpes Simplex 1 & 2 DNA Detection
HIV Quantitation
Human Herpes Virus 6 (HHV6) DNA Detection
Human Herpes Virus 8 (HHV8) DNA Detection
JC Virus DNA Detection
Norwalk Virus RNA Detection & Genotype
Parvovirus B19 DNA Detection
Shiga-like Toxin Gene (Enterohemorrhagic E. coli) DNA Detection
Varicella Zoster Virus (VZV) DNA Detection
Herpes Virus Panel (CMV, HSV, EBV, VZV, HHV-6)

Forensic Testing 402-559-7220

Genetics 402-559-8556

Available Assays:
Serpina-1 Z (p.E342K) and S (p.E264V) mutation
Factor II - Prothrombin (G20210A)
Factor V - Leiden (G1691A)
MTHFR Mutations (C677T & A1298C)
Fragile X- PCR and Southern analysis
Hemochromatosis (G845A & C187G)
Cystic Fibrosis (39 Mutations)

Pharmacogenetics 402-559-8556/402-559-7630

Carbamazepine Sensitivity (HLA B1502)
Abacavir Sensitivity (HLA B5701)