FACS Array Fluorophores Selection

Recommended Fluorophores for the FACSArray

Fluorophore  Laser   Detector 
PE
PE-Cy7 or PerCP Cy5.5
APC or Alexa647
APC-Cy7
Green  
Green
Red
Red
Yellow (585/42)
Far Red (685LP)
Red (661/16)
NIR (780/60)

Becton Dickinson recommends PE/APC as an ideal combination for optimal signal intensity and absence of optical spillover for two-color surface immunophenotyping.

Propidium iodide, 7-AAD and TO-PRO-3 dyes are compatible with the BD FACSArray system for viability testing and cell cycle analysis.