The UNMC Tissue Sciences Facility (TSF) has been established to provide basic and specialized histology, staining, immunohistochemistry, and imaging services to the research community at UNMC for studies on animal and human tissues. The TSF offers a full range of cost effective services including tissue preparation and fixing, processing, embedding, sectioning, and standard and special stains for paraffin and frozen samples. Additionally, the TSF offers both bright field and fluorescent imaging and high throughput whole slide scanning. There are many software packages available through the TSF for image analysis, reconstruction, and deconvolution as well as technical support for these programs. The resources and services at the TSF are available to all University of Nebraska personnel, as well as outside institutions and industrial users.
Dr. Geoffrey Talmon is the new director of the facility and welcomes you. Dr. Talmon is joined by experts in a variety of technologies including David Muirhead and David Wert, as well as highly qualified and skilled staff. The facility is able to perform routine work faster and more cost effectively than in individual research labs. The facility will operate on a fee for service basis and in compliance with federal regulations will charge only the cost of the service. A complete list of charges for current tests is available, and discounts can be provided on a volume basis. In addition, we have negotiated research pricing from the Nebraska Medical Center for individual special stains and routine antibodies; however, we recommend discussing pricing with the TSF supervisor when large numbers of stains are needed.
The facility has been created through valuable contributions from the Eppley Cancer Center and the Munroe-Meyer Institute, and will be administered through the Department of Pathology and Microbiology. TSF is also supported by a Center of Biomedical Research Excellence (COBRE) award through the National Center for Research Resources, NIH 5 P20 RR018788-02.
The Tissue Sciences Facility has an extensive list of services. Clients can find instructions on tissue handling and the online form via this link that they will need to fill out and bring with them before submitting samples to the facility.
The Tissue Sciences Facility is located in room 1052 on the 1rst floor of the Durham Research Center 1 on the UNMC campus. The staff is present from 8:30 AM to 4:30 PM, Monday through Friday, to receive your specimens or answer questions. Please contact us with your inquires.
Always use fresh, unused 10% normal buffered formalin (NBF).
Make sure the fixative volume is 20x the cassette volume for proper fixing.
If you are planning to perform immunohistochemistry (IHC), 24-96 hours fixing times work best.
For better brain sections, fix in 10% NBF for one week and only store in 70% ethanol for less than one day.
Fatty tissue requires longer processing times, i.e. 15 hours compared to our standard 8 hour tissue protocol.
Please consult with TSF staff regarding special types of tissues to insure proper fixing and sectioning.