Dr. Bidasee's Lab

Welcome to Dr. Keshore Bidasee's Laboratory

Collaborators
Research Goals
Funding
Techniques used in the laboratory
Personnel
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Dr. Bidasee's Biographical Information 


Collaborators:
Dr. George Rozanski, Department of Cellular and Integrative Physiology, University of Nebraska Medical Center
Dr. Channabasavaiah B Gurumurthy, Department of Genetics, Cell biology and Anatomy, University of Nebraska Medical Center
Dr. Shelby Kutty, Joint Division of Pediatric Cardiology, University of Nebraska/Creighton University and Children's Hospital and Medical Center, Omaha, Nebraska
Dr. Jaipaul Singh, School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, UK
Dr. Ryoji Nagai, Department of Food and Nutrition, Laboratory of Nutritional Science and Biochemistry, Japan Women's University 2-8-1 Mejirodai, Bunkyoku, Tokyo, Japan
Dr. Cyrus DeSouza, Department of Internal Medicine, Section of Diabetes, Endocrinology and Metabolism, ‪University of Nebraska Medical Center and Omaha VA Medical Center, Omaha, NE, USA.



Research Goals:
Effect of diabetes on expression and function of calcium cycling proteins
Long range goals:To (i) better understanding molecular mechanisms by which the activity of ryanodine receptor calcium-release channels and sarcoplasmic reticulum calcium ATPase decreases during diabetes, and (ii) identify therapeutic strategies modalities to slow the progression of this "diabetic heart muscle disease."

Significance: This research is especially important because it could lead to improvements in the quality of life for diabetic patients and help control the escalating economic cost medical expenditure and loss productivity which was estimated at $132 billion in 2002.


Funding:
Role of Ryodine Receptors in Diabetic Cardiomyopathy
PI: Bidasee, K.
NIH/NHLBI
The goals of this project are to investigate the effects of diabetes on expression and function of cardiac ryanodine receptors and to determine if changes induced by diabetes can be attenuated with drug and exercise training.

Reactive Carbonyl Species and Neurological Disorders
Nebraska Redox Biology Center


Techniques used in the laboratory

  • Generation and caring for streptozotocin-induced diabetic rats
  • Myocyteisolation
  • Video edge detection (myocyte contractility)
  • Laser confocal microscopy (calcium transients)
  • Receptor binding PCR and western blot analyses
  • Transformation/Transfection/Cloning
  • Site-directed mutagenesis
  • Lipid bilayer
  • Mass spectrometry (MALDI-TOF and Tandem)
  • Chemical synthesis
  • Echocardiography
  • Left ventricular pressure measurements (in vivo)

Personnel:

Fadhel Alomar

Fadhel Alomar
Graduate Student
falomar@unmc.edu

Hassan_Alsaad

Hassan Alsaad
Graduate Student
hassan.alsaad@unmc.edu

Poonam_Joshi

Poonam Joshi
poonam.joshi@unmc.edu

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