Welcome to Dr. Shilpa Buch's laboratory
Collaborators
Research Goals
Funding
Techniques used in the laboratory
Personnel
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Dr. Buch's Biographical Information
Collaborators
Paul Cheney, PhD, KUMC
Howard Fox, MD, PhD, UNMC
Huangui Xiong, MD, PhD , UNMC
Research Goals
The long term goal of our group is to identify novel therapeutic strategies that may enhance neuronal function and survival in NeuroAIDS, with possible implications in other neurodegenerative diseases.
Funding
Cocaine & HIV: Role of PDGF/PDGF-Receptor Axis in Blood Brain Barrier Disruption
PI: S Buch
Source: NIH/NIDA 5 R01 DA027729
Studies proposed in this grant are aimed at exploring the mechanisms of cocaine-mediated break down of blood brain barrier specifically with respect to the phosphorylation of PDGF receptor on endothelial cells.
HIV-Encephalitis and Cocaine Abuse: Mechanism of Synergy and Therapy
PI: S Buch
Source: NIH/NIDA 5R01DA020392-03
This proposal aims to: a) explore the role of cocaine in accelerating HIV-dementia and b) to develop therapeutic intervention strategies for HIV-dementia in HIV-infected cocaine abusers.
Morphine and the Neuropathogenesis of SIV in Macaques
PI: S Buch
Source: NIH/NIDA 5R01DA024442-02
The main direction of this grant is to determine the long-term of effects of morphine on the neuropathogenesis of SIV infection with respect to virus replication in the brain and effects on activation of inflammatory cells
Mechanism and Therapy of HIV Encephalitis
PI: S Buch
Source: NIH/NIMH 2R01MH068212-05A2
This grant will explore the role of virus and host factors in over-expression of CXCL10 by macrophages and will also assess the role of IP-10 in neuronal dysfunction/death associated with HIV-encephalitis and the neuroprotective role of PDGF in this process. Delivery of liposomes carrying antisense DNA plasmids will be first optimized in macrophage cell cultures followed by delivery and biodistribution in small animals such as rats. These studies will then form the basis for antisense delivery in infected macaques to interdict the disease process vaccines to prevent and/or abort CNS disease.
PLGA/Antisense IL-10: Gene Therapy for Cocaine Abusers with HAD
PI: S Buch
Source: NIH/NIDA 1R21DA023397-01A
3-D Organotypic Model of HIV-Infectable Tissue for AIDS-associated Dementia
PI: Nickerson; Co-I: S Buch
NIH/NIMH 1R21 MH080702 -01
Development of a 3D model using primary macrophages, astrocytes and neurons for
Techniques used in the laboratory
- Confocal Microscopy
- Chromatin Immunoprecipitation (ChiP) Assays
- Transmigration Assays
- In situ hybridization
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Crystal Bethel-Brown Post Doc Research Associate |
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Yeon Hee Kook |
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Fang Niu |
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Shannon Callen Research Coordinator scallen@unmc.edu |
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Yaman Lu Research Technician II yaman.lu@unmc.edu |
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Fuwang Peng |
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Xingcheng Chen |
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Rong Ma
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Lu Yang |
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Yun Gao |
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Blaise Mathias Costa |
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Honghong Yao |
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