Pawel Ciborowski, PhD







Professional Summary
Representative Publications
Biographical Information
Visit Dr. Ciborowski's Lab

Director, Mass Spectometry and Proteomics Core Facility

Durham Research Center, Room 3048
985800 Nebraska Medical Center
Omaha, NE 68198-5800


Keywords: proteomics, macrophage, HIV-1, drugs of abuse, biomarkers, histones

Meet UNMC Distinguished Scientist Pawel Ciborowski, Ph.D.

2nd International Conference on Virology, August 20-23, 2012, Las Vegas, Nevada, USA
Chair, Organizing Committee

International Conference and Exhibition on Virology, September 5-7, 2011, Baltimore, Maryland, USA
Keynote address:  “HIV-1 and Mononuclear Phagocytes: Omics Approaches to Understand Their Coexistence”

Professional Summary:
Research in the Proteomics Laboratory is focused on the correlation of structure and function of proteins, their receptors and the impact of posttranslational modifications on their functions. Unlike genes, which all use the same code, each protein has unique physical properties, preventing attempts to develop a single method to study their structure and function in living organisms.

Our effort is to use a proteomics and system biology approach supported by bioinformatics analyses to understand molecular mechanisms of pathological processes and apply this information to designing new strategies for disease prevention, early diagnosis, and control. Our primary model is the macrophage, which is a major component of the innate immunity system, and we are using state-of-the-art techniques in proteomics to investigate the following:

  • Response of macrophage to HIV-1 infection;
  • Response of macrophage to changes in cell's environment such as tissue injury; infection, toxicity of drugs used for treatment, and effect of drugs of abuse;
  • Epigenetic regulation of macrophage responses; and
  • Identification of proteomic biomarkers for the early detection and monitoring neurological complications associated with HIV-1 infection.

There are three ongoing projects in Proteomics Laboratory.

-       Project 1 is focused on changes in post-translational modifications of histones as a mechanism of regulating macrophage responses to insults and/or stimulatory molecules.

-       Project 2 is to use systems biology (holistic) experimental approaches to understand how HIV-1 is affecting macrophages to turn into machinery for productive infection allowing virus to persist in cells despite the macrophage's immune responses. Subsequently, we want to apply this information in designing new strategies for disease prevention, early diagnosis, and control. Using proteomic experiments, we will investigate mechanism(s) of how HIV-1 can bypass anti-viral intracellular immune surveillance. We are strongly interested in the effect of drugs of abuse on the function of the innate immunity system. Use of illicit drugs is an increasing social and health problem and has been documented to not only affect the brain but also the immune system making these individuals more susceptible to infections and other pathological effects. We also study the effect that anti-retroviral therapy may have on the function of macrophages.

-     Project 3 is focused on macrophages and microglia and their production of neurotoxic products that follow viral-infection and immune activation, which incite neuronal damage leading to HIV-1-neurocognitive disorders (HAND). This project is mostly focused on biomarker discovery using global proteomics profiling.

Representative Publications

  1. J. Wiederin, R. Donahoe, J. Anderson, F. Yu, H. Fox, P. Ciborowski* and H.E. Gendelman. Changes in the Plasma Proteome Follows Chronic Opiate Administration In Simian Immunodeficiency Virus Infected Rhesus Macaques. Drug and Alcohol Dependence, 2012, 120:105-12, PMCID: PMC3245805. *- corresponding author.
  2. G. Pottiez and P. Ciborowski. Elucidating gelsolin interacting domains using chemical cross-linking and MALDI-TOF/TOF mass spectrometry. Analytical Biochemistry 2012, 421:712-8. PMCID:PMC3287059.
  3. G. Pottiez, T. Jagadish, F. Yu, S. Letendre, R. Ellis, N.A. Duarte, I. Grant, H.E. Gendelman, H.S. Fox and P. Ciborowski. Plasma Proteomic Profiling in HIV-1 Infected Methamphetamine Abusers. PLoS One. 2012; 7:e31031. PMCID: PMC3281056
  4. T. Jagadish, G. Pottiez, H.S. Fox and P. Ciborowski. Plasma gelsolin accumulates in macrophage nodules in brains of Simian Immunodeficiency Virus infected rhesus macaques. J Neurovirol. 2012 Apr 19(2):113-9 [Epub ahead of print].
  5. Pottiez G, Wiederin JL, Fox H, Ciborowski P.  Comparison of 4-plex to 8-plex iTRAQ® quantitative measurements of proteins in human plasma samples.  J Proteome Res. 2012 11:3774-81 PMCID: PMC3390908. 
  6. S.D. Kraft-Terry, I. L. Engebretsen, D. K. Bastola, H. S. Fox, P. Ciborowski, and H. E. Gendelman Pulsed stable isotope labeling of amino acids in cell culture demonstrate dynamic interactions between HIV-1 and the monocyte-derived macrophage.  J. Proteome Res. 2011, 3:2852-2862. PMCID: PMC3108467.
  7. J. Wiederin, R. Donahoe, J. Anderson, F. Yu, H. Fox, H.E. Gendelman, and P. Ciborowski.  Plasma Proteomic Fingerprinting Reflects Simian Immunodeficiency Virus Infection in Rhesus Macaques. J. Proteome Res., 2010, 9:4721-4731. PMCID: PMC3285256.
  8. N. Haverland, G. Pottiez, J. Wiederin, and P. Ciborowski.  Immunoreactivity of anti-gelsolin antibody: Implications for biomarker validation.  Journal of Translational Medicine, 2010, 8:137. PMCID: PMC3019187.
  9. G. Pottiez, N. Haverland and P. Ciborowski.  Mass spectrometry characterization of gelsolin isoforms. Rapid Communications in Mass Spectrometry, 2010, 24:2620-2624. PMCID: PMC3286873.
  10. J Silberring and P Ciborowski. Biomarker discovery and clinical proteomics. Trends Analyt Chem. 2010 Feb 1;29(2):128-140. PMCID: PMC2822390
  11. J. Wiederin, F. Duan, W. Rozek and P. Ciborowski. Biomarkers of HIV-1 Associated Neurocognitive Disorders: Proteomic Investigation of Sera, Proteome Science, 2009, 7:8. PMCID: PMC2666658
  12. T. Wang, N. Gong, J. Liu, I. Kadiu, S. D. Kraft-Terry, R. L. Mosley, D. J. Volsky, P. Ciborowski and H. E. Gendelman. Proteomic Modeling for HIV-1 Infected Microglia-Astrocyte Crosstalk. PLoS ONE. 2008, 3(6):e2507. PMCID: PMC2429966
  13. W. Rozek, J. Horning, J. Anderson and P. Ciborowski. Sera Proteomic Biomarker Profiling in HIV-1 Infected Subjects with Cognitive Impairment. Proteomics – Clinical Applications, 2008, 2:1484-1507. PMCID: PMC3406605
  14. P. Ciborowski*, I. Kadiu, W. Rozek, K. Bernhardt, M. Fladseth, M. Ricardo-Dukelow, and H.E. Gendelman. Investigating the Human Immunodeficiency Type One-Infected Monocyte-Derived Macrophage Secretome.  Virology. 2007, 363:198-209, *- corresponding author. PMCID: PMC1993545
  15. I. Kadiu, M. Ricardo-Dukelow, P. Ciborowski,  and H. E. Gendelman.  Cytoskeletal protein transformation in HIV-1 infected macrophage giant cells.  J. Immunol., 2007, 178:6404-15.
  16. W. Rozek, M. Ricardo-Dukelow, S. Holloway, V. Wojna, L. Melendez, and P. Ciborowski. Cerebrospinal fluid proteomic profiling of HIV-1-infected patients with or at risk for cognitive impairments. J. Proteome Res. 2007, 6:4189-99.
  17. M. Ricardo-Dukelow, I. Kadiu-Kieken, W. Rozek, J. Schlautman, Y. Persidsky, P. Ciborowski, G. D. Kanmogne, H. E. Gendelman.  HIV-1 infected monocyte-derived macrophages affect the human brain microvascular endothelial cell proteome: New insights into blood-brain barrier dysfunction for HIV-1-associated dementia.  J. Neuroimmunol., 2007,185:37-46. PMCID: PMC2212599
  18. P. Ciborowski*and H.E. Gendelman.  Human Immunodeficiency Virus – Mononuclear Phagocyte Interactions:  Emerging Avenues of Biomarker Discovery Modes of Viral Persistence and Disease Pathogenesis.  Curr HIV Res. 2006, 4:279-91. *- corresponding author
  19. Y. Enose, C. Destache, A. Mack, J. Anderson, F. Ullrich, P. Ciborowski, and H. Gendelman.  Proteomic Fingerprints Distinguish Microglia, Bone Marrow and Spleen Macrophage Populations.  Glia. 2005, 51:161-172.
  20. P. Ciborowski*, Y. Enose, A. Mack, M. Fladseth, and H. Gendelman.  Diminished matrix metalloproteinase 9 secretion in human immunodeficiency virus infected mononuclear phagocytes: modulation of innate immunity and implications for neurological disease.  J. Neuroimmunol. 2004, 157:11-16. *- corresponding author

Additional publications in PubMed

Dr. Ciborowski's Biographical information 
Visit Dr. Ciborowski's proteomics laboratory

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