Pawel Ciborowski, PhD

 

 

 

 

 

 

 
Professional Summary
Research
Representative Publications
Biographical Information
Visit Dr. Ciborowski's Lab

PAWEL CIBOROWSKI, PhD
Associate Professor
Director, Mass Spectometry and Proteomics Core Facility

Durham Research Center, Room 3048
985800 Nebraska Medical Center
Omaha, NE 68198-5800

402-559-3733
E-mail: pciborowski@unmc.edu

Keywords: proteomics, macrophage, HIV-1, drugs of abuse, biomarkers, histones

Meet UNMC Distinguished Scientist Pawel Ciborowski, Ph.D.

2nd International Conference on Virology, August 20-23, 2012, Las Vegas, Nevada, USA
Chair, Organizing Committee

International Conference and Exhibition on Virology, September 5-7, 2011, Baltimore, Maryland, USA
Keynote address:  “HIV-1 and Mononuclear Phagocytes: Omics Approaches to Understand Their Coexistence”

Professional Summary:
Research in the Proteomics Laboratory is focused on the correlation of structure and function of proteins, their receptors and the impact of posttranslational modifications on their functions. Unlike genes, which all use the same code, each protein has unique physical properties, preventing attempts to develop a single method to study their structure and function in living organisms.

Research:
Using state-of-the-art techniques in proteomics, we are investigating the structure and function of proteins which are involved in:

  • response to changes in cell's environment,
  • response to stimuli such as tissue injury, infection, and drug treatment, and
  • during malignant transformation.

Our effort is to use a proteomics approach in understanding molecular mechanisms of pathological processes and to apply this information in designing new strategies for disease prevention, early diagnosis, and control.

There are currently three ongoing projects in the Proteomics Laboratory. Two of these projects focus on changes in proteomes of human macrophages in response to HIV-1 infection. These studies will address the hypothesis that alterations in monocyte-derived macrophage (MDM) immunity occur as a consequence of HIV-1 infection permit the virus to persist in cells despite the macrophage's phagocytic, innate and adaptive immune responses.

These studies will lead to identification of how cellular proteins are linked to the establishment of persistent viral replication in MDM are regulated. Thus, to determine the potential pathways for how HIV-1 can bypass anti-viral intracellular immune responses, proteomic tests will be used to uncover protein fingerprints of HIV-1 infected MDM (in laboratory experiments using a panel of macrophage, lymphocyte, dual and neuro-tropic viral isolates) obtained from seronegative donors.

The third project is focused on microglia and their production of inflammatory neurotoxic products that follow viral-infected and immune activation incite neuronal damage leading to HIV-1-associated dementia (HAD).

To address the question of the unique role of microglia during HIV-1 infection, we are studying proteome profiles of purified populations of bone marrow derived macrophages, splenic macrophages and microglia (adult and fetal sources) obtained from C57BL/6 mice. The second part of this project deals with electrophysiology of these cells.

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Representative Publications

  1. J. Wiederin, R. Donahoe, J. Anderson, F. Yu, H. Fox, P. Ciborowski* and H.E. Gendelman. Changes in the Plasma Proteome Follows Chronic Opiate Administration In Simian Immunodeficiency Virus Infected Rhesus Macaques. Drug and Alcohol Dependence, 2012, 120:105-12, PMCID: PMC3245805. *- corresponding author.
  2. G. Pottiez and P. Ciborowski. Elucidating gelsolin interacting domains using chemical cross-linking and MALDI-TOF/TOF mass spectrometry. Analytical Biochemistry 2012, 421:712-8. PMCID:PMC3287059.
  3. G. Pottiez, T. Jagadish, F. Yu, S. Letendre, R. Ellis, N.A. Duarte, I. Grant, H.E. Gendelman, H.S. Fox and P. Ciborowski. Plasma Proteomic Profiling in HIV-1 Infected Methamphetamine Abusers. PLoS One. 2012; 7:e31031. PMCID: PMC3281056
  4. T. Jagadish, G. Pottiez, H.S. Fox and P. Ciborowski. Plasma gelsolin accumulates in macrophage nodules in brains of Simian Immunodeficiency Virus infected rhesus macaques. J Neurovirol. 2012 Apr 19(2):113-9 [Epub ahead of print].
  5. Pottiez G, Wiederin JL, Fox H, Ciborowski P.  Comparison of 4-plex to 8-plex iTRAQ® quantitative measurements of proteins in human plasma samples.  J Proteome Res. 2012 11:3774-81 PMCID: PMC3390908. 
  6. S.D. Kraft-Terry, I. L. Engebretsen, D. K. Bastola, H. S. Fox, P. Ciborowski, and H. E. Gendelman Pulsed stable isotope labeling of amino acids in cell culture demonstrate dynamic interactions between HIV-1 and the monocyte-derived macrophage.  J. Proteome Res. 2011, 3:2852-2862. PMCID: PMC3108467.
  7. J. Wiederin, R. Donahoe, J. Anderson, F. Yu, H. Fox, H.E. Gendelman, and P. Ciborowski.  Plasma Proteomic Fingerprinting Reflects Simian Immunodeficiency Virus Infection in Rhesus Macaques. J. Proteome Res., 2010, 9:4721-4731. PMCID: PMC3285256.
  8. N. Haverland, G. Pottiez, J. Wiederin, and P. Ciborowski.  Immunoreactivity of anti-gelsolin antibody: Implications for biomarker validation.  Journal of Translational Medicine, 2010, 8:137. PMCID: PMC3019187.
  9. G. Pottiez, N. Haverland and P. Ciborowski.  Mass spectrometry characterization of gelsolin isoforms. Rapid Communications in Mass Spectrometry, 2010, 24:2620-2624. PMCID: PMC3286873.
  10. J Silberring and P Ciborowski. Biomarker discovery and clinical proteomics. Trends Analyt Chem. 2010 Feb 1;29(2):128-140. PMCID: PMC2822390
  11. J. Wiederin, F. Duan, W. Rozek and P. Ciborowski. Biomarkers of HIV-1 Associated Neurocognitive Disorders: Proteomic Investigation of Sera, Proteome Science, 2009, 7:8. PMCID: PMC2666658
  12. T. Wang, N. Gong, J. Liu, I. Kadiu, S. D. Kraft-Terry, R. L. Mosley, D. J. Volsky, P. Ciborowski and H. E. Gendelman. Proteomic Modeling for HIV-1 Infected Microglia-Astrocyte Crosstalk. PLoS ONE. 2008, 3(6):e2507. PMCID: PMC2429966
  13. W. Rozek, J. Horning, J. Anderson and P. Ciborowski. Sera Proteomic Biomarker Profiling in HIV-1 Infected Subjects with Cognitive Impairment. Proteomics – Clinical Applications, 2008, 2:1484-1507. PMCID: PMC3406605
  14. P. Ciborowski*, I. Kadiu, W. Rozek, K. Bernhardt, M. Fladseth, M. Ricardo-Dukelow, and H.E. Gendelman. Investigating the Human Immunodeficiency Type One-Infected Monocyte-Derived Macrophage Secretome.  Virology. 2007, 363:198-209, *- corresponding author. PMCID: PMC1993545
  15. I. Kadiu, M. Ricardo-Dukelow, P. Ciborowski,  and H. E. Gendelman.  Cytoskeletal protein transformation in HIV-1 infected macrophage giant cells.  J. Immunol., 2007, 178:6404-15.
  16. W. Rozek, M. Ricardo-Dukelow, S. Holloway, V. Wojna, L. Melendez, and P. Ciborowski. Cerebrospinal fluid proteomic profiling of HIV-1-infected patients with or at risk for cognitive impairments. J. Proteome Res. 2007, 6:4189-99.
  17. M. Ricardo-Dukelow, I. Kadiu-Kieken, W. Rozek, J. Schlautman, Y. Persidsky, P. Ciborowski, G. D. Kanmogne, H. E. Gendelman.  HIV-1 infected monocyte-derived macrophages affect the human brain microvascular endothelial cell proteome: New insights into blood-brain barrier dysfunction for HIV-1-associated dementia.  J. Neuroimmunol., 2007,185:37-46. PMCID: PMC2212599
  18. P. Ciborowski*and H.E. Gendelman.  Human Immunodeficiency Virus – Mononuclear Phagocyte Interactions:  Emerging Avenues of Biomarker Discovery Modes of Viral Persistence and Disease Pathogenesis.  Curr HIV Res. 2006, 4:279-91. *- corresponding author
  19. Y. Enose, C. Destache, A. Mack, J. Anderson, F. Ullrich, P. Ciborowski, and H. Gendelman.  Proteomic Fingerprints Distinguish Microglia, Bone Marrow and Spleen Macrophage Populations.  Glia. 2005, 51:161-172.
  20. P. Ciborowski*, Y. Enose, A. Mack, M. Fladseth, and H. Gendelman.  Diminished matrix metalloproteinase 9 secretion in human immunodeficiency virus infected mononuclear phagocytes: modulation of innate immunity and implications for neurological disease.  J. Neuroimmunol. 2004, 157:11-16. *- corresponding author

Additional publications in PubMed

Dr. Ciborowski's Biographical information 
Visit Dr. Ciborowski's proteomics laboratory

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