Dr. Ciborowski's Lab

Welcome to Dr. Pawel Ciborowski's Proteomics Laboratory

Collaborators
Funding
Techniques used in the laboratory
Personnel
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Dr. Ciborowski's biographical information
 


Collaborators
Mike Boska, PhD, Radiology, UNMC
Howard E. Gendelman, MD, Pharmacology and Experimental Neuroscience, UNMC
Howard S. Fox, MD, PhD, Pharmacology and Experimental Neuroscience, UNMC
R. Lee Mosley, PhD, Pharmacology and Experimental Neuroscience, UNMC
Huangui Xiong, M.D., PhD, Pharmacology and Experimental Neuroscience, UNMC
Georgette Kanmogne, PhD, Pharmacology and Experimental Neuroscience, UNMC
Jialin Zheng, MD, Pharmacology and Experimental Neuroscience, UNMC
Keshore Bidasee, PhD, Pharmacology and Experimental Neuroscience, UNMC
James Turpen, PhD, Genetics Cell Biology & Anatomy, UNMC
Mike Belshan, PhD, Creighton University, Omaha, NE
Igor Grant, MD, University of California, San Diego
Ronald Ellis, MD, PhD, University of California, San Diego
Scott Letendre, MD, University of California, San Diego
Loyda Melendez, PhD, University of Puerto Rico Medical Science Campus. Dr. Juliana Perez-Laspiur, University of Puerto Rico Medical Science Campus


Funding "Neuroprotective Immunity and HIV Dementia"

PI: H. E. Gendelman; Co-I: P. Ciborowski
Source: NIH/NINDS
This proposal will determine cell responses in macrophages following HIV-1 infection and engagement with T cells and T cell subsets. Macrophage functions including phagocytosis, antigen presentation, intracellular killing and effector cell responses and their modulation by T cells is a focus for this work. Signal transduction pathways and mechanisms for virus-induced neurotoxicity or neuroprotection will be developed.

“Neuroprotective Immunity and HIV Dementia”
PI: H. E. Gendelman; Co-I: P. Ciborowski
Source: NIH/NINDS
This proposal will determine cell responses in macrophages following HIV-1 infection and engagement with T cells and T cell subsets. Macrophage functions including phagocytosis, antigen presentation, intracellular killing and effector cell responses and their modulation by T cells is a focus for this work. Signal transduction pathways and mechanisms for virus-induced neurotoxicity or neuroprotection will be developed.

“Neural Immunity in HIV Dementia”
PI: H. E. Gendelman, Proteomics Core Leader: P. Ciborowski
Source: NIH/NINDS
The major goal of this grant is the study of the specific immunologic basis of HAD and linkages between it and other neurodegenerative disorders. This grant brings together in one organizational structure a group of scientists with expertise in areas of neurotoxicology, cellular immunology, neuropathology, neurophysiology, neuropharmacology and molecular biology, who maintain a unified focus on how MP biology affects both neurodegeneration and neuroprotection.

"Role of Ryodine Receptors in Diabetic Cardiomyopathy"
PI: K. Bidasee; Co-I: P. Ciborowski
Source: NIH/NHLBI
The goals of this project are to investigate the effects of diabetes on expression and function of cardiac ryanodine receptors and to determine if changes induced by diabetes can be attenuated with drug and exercise training.

"Blood Brain Barrier Immune Compromise in NeuroAIDS"
PI: G. Kanmogne; Co-I: P. Ciborowski
Source: NIH/NIMH
The major goal of this project is to provide insights into the mechanisms by which cytokines and HIV-1 transduce signals at the blood-brain barrier (BBB), dysregulations that occurs in HIV infection and lead to BBB dysfunction

"SDF-1 and Neurogenesis in HIV-1 Associated Dementia"
PI: J. Zheng; Co-I: P. Ciborowski
Source: NIH/NINDS
The major goals of this project are to investigate the role of SDF-1 and CXCR4 mediated neuronal repair process in HIV-1 associated dementia.

“Proteomic Strategies for AIDS and Drug Abuse – HIV and METH CNS Synergy”
PI: H. Fox; Project 3 Leader: P. Ciborowski
Source: NIH/NIDA
The aims of the Program Project are to develop and use proteomic strategies to uncover the aspects of methamphetamine, HIV-1 and glial crosstalk (Project 1), methamphetamine and the neuropathogenesis of SIV infection (Project 2), and biomarkers in an HIV-1 infected drug abusing clinical cohort (Project 3).

“Macrophages, NR2B-containing NMDA receptors and HIV dementia”
PI: H. Xiong; Co-PI: P. Ciborowski
Source: NIH/NINDS
This proposal investigates how HIV-1-infected mononuclear phagocytes (brain macrophages and microglia) activate neuronal extra-synaptic NR2BRs, leading to neuronal damage and ultimately neurocognitive dysfunction.

“Nebraska Research Network in Functional Genomics”
PI: J. B. Turpen; Mass Spectrometry and Proteomics Core Director: P. Ciborowski
Source: NIH/NCRR
The goal of this project is to support and foster biomedical research in undergraduate universities in Nebraska. return to top of page


Techniques used in the laboratory

  • Proteomics: MudPIT, 2-dimensional SDS-PAGE with DIGE, ProteomeLab PF2D, iTRAQ
  • Mass Spectrometry: nano-LC-MS/MS ion traps, triple quads, MALDI-TOF/TOF
  • Biochemistry: ELISA, western blot, immunopurification
  • Tissue Culture: Cell cultures and cell based assays
  • Virology: HIV-1 in vitro infections
  • Immunology: cytokines

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Personnel

Ariel_Burns  Ariel Burns
Graduate Student
ariel.burns@unmc.edu
  Teena_Jagadish

Teena Jagadish
Research Technologist
tjagadish@unmc.edu

         
Nicky_Haverland Nicole Haverland
Graduate Student
nhaverland@unmc.edu
   Jayme_Wiederin

Jayme Wiederin, MS
Researcher
jhorning@unmc.edu

         
Tomas_Helikar Tomas Helikar
Research Associate
   Jessica_Wooden

Jessica Wooden
Research Technologist
jessica.wooden@unmc.edu


Former Lab Members
Pawel Olsowy
Gwen Pottiez
Wojtek Rozek

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Pawel Ciborowski biographical information 

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