Dr. Gendelman's Lab

Welcome to Dr. Howard Gendelman's Neuroregeneration Laboratory

Research Goals
Techniques used in the laboratory
Return to Dr. Gendelman's home page
Dr. Gendelman's Biographical Information


Michele Aizenberg, Neurosurgery, University of Nebraska Medical Center (UNMC), Omaha, NE

Mike Boska, Radiology UNMC, Omaha, NE

Tatiana Bronich, Center for Drug Delivery and NanoMedicine, Department of Pharmaceutical Sciences

R. Lee Mosley, Pharmacology and Experimental Neuroscience, UNMC, Omaha, NE

Stephen Dewhurst, University of Rochester, Rochester, NY

Harris Gelbard, University of Rochester School of Medicine, Rochester, NY

Edmundo Kraiselburd, University of Puerto Rico, San Juan, PR

Serge Przedborski, Columbia University, New York, NY

David George Standaert, University of Alabama-Birmingham, Birmingham, AL

David Volsky, St. Luke's Roosevelt Hospital, New York, NY

Charles Wood, Nebraska Center for Virology, UNL, Lincoln, NE

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Research Goals

The long-range goals of the laboratory are to develop immunopharmacological approaches to improve the diagnosis and treatment options for neurodegenerative diseases that include HIV-1 associated neurocognitive disorders (HAND) and Parkinson's disease (PD).

The laboratory initiative is divided into specific programs, each co-headed by an experienced scientist. Specific expertise in immunology, molecular biology, infectious disease, proteomics, physiology and pathogenesis is provided to the student and research fellow.

First, studies revolving around the development of nanoformulated cell-based drug delivery with a special focus to the CNS. Nanotoxicology studies involve the studies of the regulation of leukocyte entry into peripheral and neural tissues with a focus on glial immunity. This is seen during neurodegenerative diseases and include studies of HAND and PD. Those that are being developed include, but are not limited to, antiretroviral, neuroprotective, and anti-inflammatory drugs.

Second, drug testing (neuroprotective and neuroregenerative) and improved diagnostics for HAND is being developed in virus-infected immunodeficient mice. This program is part of national grant efforts that involve scientists at the University of Nebraska Medical Center, the University of Rochester, and Columbia University College of Physicians and Surgeons. The focus is to perform translational research that would move quickly from animals to humans.

Third, proteomic and metabolomic approaches for biomarker discovery are linked to studies of cell-cell interactions for disease. This is part of a broader initiative in studies of how drugs of abuse influence viral replication and immune dysregulation.

Fourth, neuroimmunologic and vaccine approaches that induce protective immunity and neuroregeneration are being pursued in animal models of HAND, PD and amyotrophic lateral sclerosis. The laboratory has developed a unique immunization approach that uses the immune system as a cell source for brain repair during neurodegenerative.

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PI: Gendelman, Howard E.

NanoART Manufacture, Delivery and Pharmacokinetics for Optimizing Drug Adherence
PI: Gendelman, H. E.
This is an integrative cross approach translational and multi-investigator program grant seeking to develop nanoformulated antiretroviral drug therapy from the bench to the patient.

Neural Immunity in HIV Dementia
PI: Gendelman, H. E.
The major goal of this grant is the study of the specific immunologic basis of HAD and linkages between it and other neurodegenerative disorders. This grant brings together in one organizational structure a group of scientists with expertise in areas of neurotoxicology, cellular immunology, neuropathology, neurophysiology, neuropharmacology and molecular biology, who maintain a unified focus on how MP biology affects both neurodegeneration and neuroprotection.

Neuroprotective Immunity and HIV Dementia
PI: Gendelman, H. E.
This proposal will determine cell responses in macrophages following HIV-1 infection and engagement with T cells and T cell subsets. Macrophage functions including phagocytosis, antigen presentation, intracellular killing and effector cell responses and their modulation by T cells is a focus for this work. Signal transduction pathways and mechanisms for virus-induced neurotoxicity or neuroprotection will be developed.

Nanomedicine and NeuroAIDS
PI: Gendelman, H. E.
This research proposes to investigate the biophysiological properties of monocytes and monocyte-derived macrophages that influence cell migration both across the blood-brain barrier and within the brain. The central hypothesis is that changes in ion channel expression in monocytes and macrophages following exposure to virus and immune products influences the cell's ability to change its volume and shape, thus influencing cell migration. Such events are pivotal for macrophages to enter the brain and to secrete the toxins that underlie the neuropathogenesis of HIV-1 associated dementia.

Targeted Neuroprotection for HIV-1 Associated Neurologic Disease
PI: Gelbard, H.; Project 2 Leader: Gendelman, H. E. (Project 2: Novel Adjunctive Therapies for NeuroAIDS)
The purpose of this core is to assist in the design of adjunctive therapeutic strategies for treatment and/or prevention of neurologic disease following human immunodeficiency virus type-one (HIV-1) infection. In this regard, purified human monocyte-derived macrophages (MDM) and MDM conditioned media (MCM) will be supplied, following HIV-1 infection and/or immune activation, to all investigators on request, within the Rochester Cooperative NeuroAIDS Drug Discovery Group (RCNDDG). In addition, promising anti-inflammatory and/or neuroprotective drugs, developed in laboratory assays or proposed, will be tested for therapeutic efficacy in a severe combined immune deficiency (SCID) mouse model of HIV-1 encephalitis (HIVE).

Nebraska Center for Nanomedicine
PI: Bronich, T; Co-I: Gendelman, H. E.
This proposal seeks to develop an interdisciplinary Nanomedicine Center at the University of Nebraska Medical Center (UNMC). The focus of this proposal is to develop the means to best use devices of nanoscale size to improve outcomes for cancer, neurodegenerative and cardiovascular diseases.

Chronic HIV infection and Aging in NeuroAIDS (CHAIN) Center
PI: Fox, H.; Co-I: Gendelman, H. E.
This is a Center grant to provide Administrative and Core Support for scientists investigating NeuroAIDS.

T Cell-Mediated Neurodegeneration in Parkinson's Disease
PI: Mosley, R.L; Co-I: Gendelman, H. E.
This grant proposes to elucidate the role of T cell-mediated immunity in the pathogenesis of disease progression in a murine model of Parkinson's disease. We hypothesize that the tempo and progression of PD is accelerated by N-a-syn stimulation of microglia and APCs, recognition by the adaptive immune system of neoantigenic epitopes on N-a-syn, and induction of effector T cells (Teffs) that extravasate to neuroinflammatory sites, exacerbate inflammatory microglia, and augment microglia-mediated neurotoxicity. To test our hypothesis, 3 proposed aims will determine 1) the N-a-syn epitopes and MHC elements permissive for induction of T helper cells that mediate dopaminergic neurodegeneration, 2) N-a-syn-specific T helper effector subset(s) responsible for exacerbated neurodegeneration, and 3) molecular and biochemical mechanisms by which N-a-syn specific Teff subset(s) modulate neurodegeneration.

An Integrated Immune, Biomaterial and Stem Cell Platform for Neuroprotection and Neuroregeneration Designed to Improve Outcomes for Battlefield Injuries
PI: Kabanov A; Co-I:  Gendelman HE
Iowa State University

MCAM Antibodies Effect on Th17 Migration and Neuroprotection in MPTP-Intoxicated Mice
Neotope Biosciences Limited
PI: Gendelman, Howard E.

Modeling monocyte and macrophage based gene therapy for neuroAIDS
University of Hawaii
PI:  Lu, Yuanan; PI, UNMC: Gendelman, Howard E.
This proposal aims to use two humanized mouse models of neuroAIDS to test the abilities of altering monocyte-macrophage migration to the nervous system as a therapeutic means to positively affect the course of disease.

Intraceullular Compartmental Kinetics of HIV Drug
University of Minnesota
PI: Fletcher; Co-I: Gendelman Howard E.

Nebraska Center for Virology
PI: Wood C; Co-I: Gendelman, Howard E.
The major goals of this grant are to develop a comprehensive center for the study of viral diseases headquartered in Lincoln Nebraska.  This is a combined and shared initiative between the University of Nebraska at Lincoln, Creighton University and the University of Nebraska Medical Center.  The moneys are used solely for developing new investigators and for recruitment.

Cell Targeted long-acting nanoformulated antiretroviral therapy (Research Collaboration Agreement-UNeMed)
ViiV Healthcare Limited
PI: Gendelman, Howard E.
These studies aim to develop formulation platforms for a long-acting integrase, a CCR5 inhibitor and a nucleoside reverse transcriptase inhibitor for once monthly therapies with reduced injection volumes and viral reservoir targeting.

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Techniques used in the laboratory

Chimeric Mice Nano-medicine macrophage delivery
Fluorescent microscopy Pathology analysis
HIV-1 neurotoxicity assay Primary brain and immune cell culture
Immunohistochemistry Real-time PCR
Macrophage migration tracking Small animal model drug therapy
Mouse intracerebral stereotactic inoculation Western blotting
Mouse models of HIV-1 encephalitis  

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Mariluz Arainga Ramirez, PhD
Post-Doc Research Associate



Aditya Bade

Aditya Bade
Graduate Student
Drs. Gendelman, Mike Boska and Yutong Liu's laboratories


Kathy Estes Kathy Estes
Research Coordinator

JoEllyn McMillan, PhD
Assistant Professor


Hannah Baldridge
Research Technologist I


James Hilaire
Graduate Student


Christine Morrison
Research Technologist I

Shantanu Balkundi

Shantanu Balkundi, PhD
Senior Research Associate


Lindsey Kendrick
Research Technologist I


 Praba Narayanasamy, PhD

Prasanta Dash

Prasanta Dash, PhD
Senior Research Associate

Tomomi Kiyota Tomomi Kiyota, PhD

Kate Olson
Graduate Student


Nicholas DeKover
MD/PhD Student


Lisa Kosloski Bilek

Graduate Student

Pavan Puligujja
Graduate Student

Benson_Edagwa Benson Edagwa
Post Doc Research Associate

 Tianyuzi Li
Graduate Student


Lana Reichardt
Administrative Assistant

 Bret Elliott
Research Technologist I
 Xinming Liu

Xinming Liu, PhD
Assistant Professor


Nate Smith
Research Technologist I nathana.smith@unmc.edu

 Adrian Epstein Adrian Epstein
MD/PhD Student
Andrea Martinez-Skinner Andrea Martinez-Skinner
Graduate Student
 Gang Zhang

Gang Zhang
Graduate Student

Return to Dr. Gendelman's home page
Dr. Gendelman's Biographical Information

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