WELCOME TO JAMES HAORAH'S LABORATORY
Collaborators
Research Goals
Funding
Techniques used in the laboratory
Personnel
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Yuri Persidsky, MD, PhD, Temple University, Philadelphia, PA
Terrence M Donohue, PhD
To study the mechanisms of alcohol-induced mitochondrial dysfunction and CNS injury
The mechanisms of cellular anti-oxidants and oxidants imbalance due to mitochondrial dysfunction will be studied. This includes the role of phospholipases A2 (PLA2), fatty acids synthesis, mitochondrial b-oxidation, and restoration of mitochondrial function by acetyl-L-carnitine or oenzyme Q10.
To study the mechanisms of neuroinflammation mediated by COX/LOX metabolism of fatty acids
The focus of this study is on the idea that EtOH-induced impairment of mitochondrial b-oxidation function enhances fatty acids (arachidonic acid, strearic acid, palmitic acid) accumulation leading to increased neuroinflammation. Signaling pathways of interest are cyclooxygenase and lipoxygenase for the production of excess inflammatory mediators such as prostaglandin E2 (PGE2) or leukotrienes (LTB4).
To study the mechanisms of neurotoxicity by fatty acid ethyl esters in alcoholics
The idea that defective mitochondrial b-oxidation and accumulation of fatty acids in the settings of alcohol abuse and oxidative stress increases the rate of fatty acids conjugation with un-metabolized ethanol forming the neurotoxic fatty acid ethyl esters products will be studied. These studies involve analytical chemistry employing the knowledge of solid-phase extraction methods, GC-MS, GC-FID, and HPLC detection techniques
Alcohol Abuse and Blood-Brain Barrier Dysfunction: Underlying Mechanisms
PI: James Haorah
Source of Funding: NIH/NIAAA (1R21 AA016403-01A2)
Alcohol Abuse and HIV-1 infection: Mechanisms of Combined CNS Injury and Interventions
Multiple PIs: James Haorah and Yuri Persidsky
Source of Funding: NIH/NIAAA (1 R01 AA017398-01)
Techniques used in the laboratory
- In vitro and animal models for Blood-Brain Barrier
- Biochemistry technologies
- Confocal Imaging facility
- Molecular biology
- Immunohistochemistry
- Real-time PCR and Western blot analyses
- Real-time transendothelial electrical resistance (TEER) by 1600R ECIS system.
- Radio-labeled tracer molecules
- Detection of fatty acid ethyl esters by GC-MS
- Detection of fatty acids by HPLC
- Detection of fluorescent tracer marker by SpectraMax M5E
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Mohammad Abdul Muneer, PhD Senior Research Associate mabdulmu@unmc.edu |
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Vikas Mishra, PhD |
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Heather A. Schuetz
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Dr. Haorah's biographical information
