Dr. Kanmogne's Lab

Welcome to Dr. Georgette Kanmogne's Laboratory

Collaborators
Research Goals
Funding
Techniques used in the laboratory
Personnel
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Dr. Kanmogne's biographical information


Collaborators
Alfred K. Njamnshi, MD, MA, DMS
Associate Professor
Chief Neurology Department. Yaoundé Central Hospital
Vice Dean, University of Yaoundé I, Cameroon

Callixte Kuate, MD
Neurologist, Yaoundé Central Hospital
Lecturer, University of Yaoundé I, Cameroon

Dora Mbanya, MD, PhD
Professor
Chief of Hematology Lab, Yaoundé Teaching Hospital, Yaoundé Central Hospital, University of Yaoundé I, Cameroon

Ronald J. Ellis, MD, PhD
Professor
HIV Neurobehavioral Research Center, University of California San Diego

UNMC Collaborators:
Howard E. Gendelman, M.D.
Larisa Poluektova, M.D., Ph.D.
Pawel Ciborowski, Ph.D.

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Research Goals
HIV-1 invades the brain in the early stages of infection and productively infects brain mononuclear phagocytes (MPs: macrophages and microglia). The virus, secreted proteins “virotoxins” and inflammatory cytokines/chemokines produced by infected MPs are thought to induce neuronal dysfunction and HIV-associated dementia (HAD). To elucidate the pathogenesis of HAD, it is important to understand by what mechanisms HIV-1 invades the brain.

Our laboratory is currently using different experimental paradigms to decipher the mechanisms mediating in blood-brain barrier (BBB) dysfunction and virus invasion of the central nervous system (CNS) in HAD. These approaches include specific cytotoxic effects of HIV-1 and virotoxins on the brain endothelium (including functional alterations of cytoskeleton and tight junction proteins) leading to increased BBB permeability, role of chemokine receptors and events occurring within endothelial cells during interaction with HIV-1 infected cells.

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Funding

"Blood Brain Barrier Immune Compromise in NeuroAIDS"
PI: G Kanmogne
NIH/NIMH 5 R01 MH081780-02
The major goal of this project is to provide insights into the mechanisms by which cytokines and HIV-1 transduce signals at the blood-brain barrier (BBB), dysregulations that occurs in HIV infection and lead to BBB dysfunction.

"NeuroAIDS in Cameroon: Molecular Determinant"
PI: G. Kanmogne
NIH/NINDS 5 R21 MH080611-02
The major goal of this project is to characterize the epidemiology of neuroAIDS in Cameroon and its molecular determinants. Specifically, the objective of this pilot study is to adapt, normalize and implement in Cameroon neuromedical and neurobehavioral test procedures, demonstrate the feasibility of identifying and recruiting participants, collecting and transferring samples to UNMC for molecular testing.

"Neural Immunity in HIV Dementia"
PI: H. Gendelman; Project 3 Leader: G. Kanmogne
NIH/NINDS 5 P01 NS043985-07
The major goal of this grant is the study of the specific immunologic basis of HAD and linkages between it and other neurodegenerative disorders. This grant brings together in one organizational structure a group of scientists with expertise in areas of neurotoxicology, cellular immunology, neuropathology, neurophysiology, neuropharmacology and molecular biology, who maintain a unified focus on how MP biology affects both neurodegeneration and neuroprotection.

"University of Rochester" -Project 2: Novel Adjunctive Therapies for NeuroAIDS
PI: H Gelbard; Co-I: H Gendelman and G Kanmogne
The purpose of this core is to assist in the design of adjunctive therapeutic strategies for treatment and/or prevention of neurologic disease following human immunodeficiency virus type-one (HIV-1) infection. In this regard, purified human monocyte-derived macrophages (MDM) and MDM conditioned media (MCM) will be supplied, following HIV-1 infection and/or immune activation, to all investigators on request, within the Rochester Cooperative NeuroAIDS Drug Discovery Group (RCNDDG). In addition, promising anti-inflammatory and/or neuroprotective drugs, developed in laboratory assays or proposed, will be tested for therapeutic efficacy in a severe combined immune deficiency (SCID) mouse model of HIV-1 encephalitis (HIVE).
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Techniques used in the laboratory

  • Tissue culture
  • Immunohistochemistry/ Laser Confocal Microscopy
  • Cell Migration Assays
  • Southern & Western blot analysis; Autoradiography
  • ELISA, PCR, RT-PCR, Real -Time PCR

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Personnel

Post-docs

Biju_Bhargavan

Biju Bhargavan, PhD
Post-Doc Research Associate

bbhargavan@unmc.edu

   
 Hong_Li

Hong Li
Research Technologist II

hongli@unmc.edu

   
 Sangya_Singh

Sangya Singh
Research Technologist I

sangyasingh@unmc.edu

   
Shawna Woollard

Shawna Woollard
Graduate Student

swoollar@unmc.edu

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Return to Dr. Kanmogne's home page
Dr. Kanmogne's biographical information

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