HuiPan

Research Interests
Representative Publications
Biographical Information

HUI PAN, PhD
Instructor
Howard Fox laboratory
Durham Research Center 3011
985800 Nebraska Medical Center
Omaha, NE 68198-5800

Phone: 402-559-2054
E-mail: hpan@unmc.edu

Keywords: neurodegenerative disease, proteomics, mass spectrometry, quantitative, neuron, autophagy, synapse, lentivirus, pathology, ubiquitin


Research Interests

My research focus aims at elucidating the common mechanisms underlying neurodegenerative diseases as well as molecular changes specific to different type of neurological diseases. One specific focus is to study how autophagy process is disrupted or disturbed in CNS under disease conditions. Due to the complex nature of the question, modern quantitative proteomics techniques in combination with conventional biochemistry and cell biological approaches will be used to tackle the question.

Long-range Goals 

Understanding what molecular pathways/signaling events related to autophagy process are disrupted or altered under neurodegenerative disease conditions

  • Whether change in autophagy dynamics is beneficial or detrimental to neurological conditions are still controversial, particularly given the complex nature of most chronic neurological diseases. Elucidating that under specific disease conditions will help to develop effective therapeutic interventions

Developing quantitative proteomics analysis pipeline including SILAC and iTRAQ for studying molecular changes in neurodegenerative disease

  • Unbiased analyses of synaptic proteins on large scale will provide interesting new leads toward understanding neurodegenerative disease process

Representative Publications

  1. Pan H., Che F-Y, Peng B., Pintar J.E., and Fricker L.D. "The Role of Prohormone Convertase-2 in Neuropeptide Processing: A Quantitative Peptidomic Study", J Neurochem. 2006 Sep;98(6):1763-77
  2. Pan H., Che F-Y, Peng B., Pintar J.E., and Fricker L.D. "The Role of Prohormone Convertase-2 in Neuropeptide Processing: A Quantitative Peptidomic Study", J Neurochem. 2006 Sep;98(6):1763-77
  3. Pan H., Che F-Y, Peng B., Pintar J.E., and Fricker L.D. "The Role of Prohormone Convertase-2 in Neuropeptide Processing: A Quantitative Peptidomic Study", J Neurochem. 2006 Sep;98(6):1763-77
  4. Pan H., Nanno D., Che F-Y, Zhu X., Salton S.R., Steiner D.F., Fricker L.D., Devi L.A. "Neuropeptide Processing Profile in Mice Lacking Prohormone Convertase-1", Biochemistry 2005 Mar 29;44(12):4939-4948
  5. Che F-Y., Lim J., Pan H., Biswas R., and Fricker L.D. "Quantitative neuropeptidomics of microwave-irradiated mouse brain and pituitary", Mol Cell Proteomics. 2005 Sep;4(9):1391-405
  6. Mzhavia N., Pan H., Che F-Y, Fricker L.D., Devi L.A. "Characterization of Endothelin Converting Enzyme-2: Implication for a Role in the Non-classical Processing of Regulatory Peptides", J Biol Chem. 2003 Apr 25;278(17):14704-11 

Techniques used in the laboratory

  • Targeted protein-protein interaction characterization using one-dimensional SDS-PAGE separation and tryptic digestion of specific protein bands of interest followed by liquid chromatography-mass spectrometry (LC-MS) analysis
  • Stable Isotope Labeling with Amino acids in Cell culture (SILAC)-for cell line studies
  • Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-for studying tissue extracts from disease animal models
  • Lentiviral transduction system-used to deliver genes of interest with temporal and spatial control to animal model through stereotaxic injection or directly applying it to primary neuronal/astroglial culture.

More Information on Dr. Pan

Visit Howard Fox laboratory

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