The aims of this National Institute of Drug Abuse Program Project are to develop and use proteomic strategies to uncover the aspects of methamphetamine (Meth), HIV-1 and glial crosstalk (Project 1), meth and the neuropathogenesis of SIV infection (Project 2), and biomarkers in an HIV-1 infected drug abusing clinical cohort (Project 3).
The grant consists of three projects and one core.
- Project 1: Meth, HIV-1 and Glial Crosstalk
- Project 2: Meth and the neuropathogenesis of SIV infection
- Project 3: Biomarkers in an HIV infected drug abusing clinical cohort
- Core A: Administrative, Proteomics and Biostatistics
Project 1: Meth, HIV-1 and Glial Crosstalk will
- uncover the HIV-1 infected MP proteome as it is influenced by Meth, and
- investigate how Meth influences the MP proteome as a consequence of glial cross talk and resultant neurotoxicity
- determine how Meth administration alters the proteome in the brains of SIV-infected monkeys and
- investigate the relationship between the protein changes, identified above with upstream changes in gene expression and downstream metabolic products.
- determine differences in proteome profiles of plasma which reflect short- and long-term withdrawal of Meth, and
- substantiate the relevance of uncovered biomarkers in molecular mechanisms of the synergistic effect of HIV-1 infection and use of Meth.
- contribute to the development of the Center's research themes through collaboration and communication
- provide focused resources to the projects, and
- educate, train and mentor the next generation of research scientists
Proteomics Core will
- provide analytical technologies in proteomics and will include resources of the Scripps Center for Mass Spectrometry directed by Dr. Gary Siuzdak and the Mass Spectrometry and Proteomics Core Facility at UNMC directed by Dr. Pawel Ciborowski
- provide experimental design and analysis support as it relates to proteomics and mass spectroscopy, and
- provide leadership and coordinate work among the program leaders on proteomic studies of molecular mechanisms underlying pathogenesis of neuroAIDs and Meth use
Gary Siuzdak, PhD
Biostatistics Core will
- provide pertinent, reliable and convincing analysis of samples obtained and used in the proteomic research
- guide statistical design of experiments for data collection
- provide statistical advice and preliminary analysis of high and low-throughput experimental data, and will
- work with investigators to apply additional analysis methods