Lyudmila S. Shlyakhtenko, Ph.D.

Research Associate Professor

Co-Director, the UNMC Nanoimaging Core Facility
Department of Pharmaceutical Sciences
College of Pharmacy
University of Nebraska Medical Center
986025 Nebraska Medical Center
Omaha, NE 68198-6025
402-559-1974 (Office)
402-559-2753 (Core Facility)
402-559-9543 (Fax)
lshlyakhtenko@unmc.edu
http://www.unmc.edu/pharmacy/nanoimaging/

Research Interests:
Dr. Shlyakhtenko's main research interest is the study structure, dynamics and interactions of various biomolecular systems by the use of Atomic Force Microscopy (AFM) as an imaging tool. Development of immobilization approaches and surface chemistry is required for successful use of AFM.  One of the most promising approaches utilizes silatrane surface chemistry.  This approach allows studying the structures of different protein/DNA complexes, unusual DNA and RNA structures, the structures of proteins, macromolecular ensembles, and different types of carriers for drug delivery.

Recent publications:

  1. Lyubchenko, Y.L. and Shlyakhtenko, L.S. (2009) AFM for analysis of structure and dynamics of DNA and protein-DNA complexes. Methods, 47, 206-213.
  2. Lyubchenko, Y.L., Shlyakhtenko, L.S. and Gall, A.A. (2009) Atomic force microscopy imaging and probing of DNA, proteins, and protein DNA complexes: silatrane surface chemistry. Methods Mol Biol, 543, 337-351.
  3. Shlyakhtenko, L.S., Lushnikov, A.Y. and Lyubchenko, Y.L. (2009) Dynamics of nucleosomes revealed by time-lapse atomic force microscopy. Biochemistry, 48, 7842-7848.
  4. Ghosh, R.P., Horowitz-Scherer, R.A., Nikitina, T., Shlyakhtenko, L.S. and Woodcock, C.L. (2010) MeCP2 binds cooperatively to its substrate and competes with histone H1 for chromatin binding sites. Mol Cell Biol, 30, 4656-4670.
  5. Zaremba, M., Owsicka, A., Tamulaitis, G., Sasnauskas, G., Shlyakhtenko, L.S., Lushnikov, A.Y., Lyubchenko, Y.L., Laurens, N., van den Broek, B., Wuite, G.J. et al. (2010) DNA synapsis through transient tetramerization triggers cleavage by Ecl18kI restriction enzyme. Nucleic Acids Res, 38, 7142-7154.
  6. Liu, J., Guo, S., Cinier, M., Shlyakhtenko, L.S., Shu, Y., Chen, C., Shen, G. and Guo, P. (2011) Fabrication of stable and RNase-resistant RNA nanoparticles active in gearing the nanomotors for viral DNA packaging. ACS Nano, 5, 237-246.
  7. Lyubchenko, Y.L., Shlyakhtenko, L.S. and Ando, T. (2011) Imaging of nucleic acids with atomic force microscopy. Methods. 54(2), 274-283.
  8. Shlyakhtenko, L.S., Lushnikov, A.Y., Li, M., Lackey, L., Harris, R.S. and Lyubchenko, Y.L. (2011) Atomic force microscopy studies provide direct evidence for dimerization of the HIV restriction factor APOBEC3G. J Biol Chem, 286, 3387-3395.
  9. Shlyakhtenko, L.S., Lushnikov, A.Y., Miyagi, A. and Lyubchenko, Y.L. (2012) Specificity of binding of single-stranded DNA-binding protein to its target. Biochemistry, 51, 1500-1509.
  10. Shlyakhtenko, L.S Lushnikov, A.Y., Miyagi, A.,Li, M., Harris, R.S., Lyubchenko, Y.L. (2012) Nanoscale structure and dynamics of APOBEC3G complexes with single-stranded DNA. Biochemistry, 51, 6432-6440.

Lyudmila S. Shlyakhtenko, Ph.D.