Yazen Alnouti, Ph.D.

Yazen Alnouti, Ph.D.

AlnoutiAssistant Professor
Department of Pharmaceutical Sciences
College of Pharmacy
University of Nebraska Medical Center
986025 Nebraska Medical Center
Omaha, NE 68198-6025
402-559-4631(Office)
402-559-2407 (Lab)
402-559-9543 (Fax)
yalnouti@unmc.edu

Teaching Activities:
At the professional level, Dr. Alnouti lectures in the areas of biopharmaceutics and pharmacokinetics in the "Introduction to Pharmaceutical Sciences" and "Pharmaceutical Sciences II & III" courses.  At the graduate level, Dr. Alnouti provides lectures in "Advanced Pharmacokinetics and Pharmacodynamics" and the "Quantitative Pharmaceutical Analysis" courses.

Research Activities/Interests:
Research in Dr. Alnouti's laboratory is involved in the multidisciplinary area of drug metabolism and pharmacokinetics (DMPK).  His research focuses on the application of bioanalytical chemistry, in vitro models such as microsomes and stably-transfected cell lines, and in vivo animal models such as knock-out mice, to support ADMET (absorption, distribution, metabolism, excretion and toxicity) and pharmacokinetics studies. This is a technique-driven enterprise; therefore expertise in liquid chromatography-mass spectrometry (LC-MS) is heavily used in his research.  

Another area of interest in Dr. Alnouti's laboratory is the study of bile acid metabolism, homeostasis, and toxicity, with particular interest in the role of sulfation, a phase II metabolic pathway, by the sulfotransferase (SULT) enzymes in controlling bile acid homeostasis.

Recent Publications:

  1. Alnouti, Yazen; Srinivasan, Karthik; Waddell, David; Bi, Honggang; Kavetskaia, Olga; Gusev, Arkady I; Development and Application of a New On-line SPE System combined with LC-MS/MS Detection for High Throughput Direct Analysis of Pharmaceutical Compounds in Plasma. Journal of Chromatography A., 1080 (2): pp. 99-106. Jul 2005
  2. Alnouti, Yazen; Li, Ming; Kavetskaia, Olga; Bi, Honggang; Hop, Cornelius; Gusev, Arkady I.; Method for Internal Standard Introduction for Quantitative Analysis Using On-Line Solid-Phase Extraction LC-MS/MS.; Analytical Chemistry, 78 (4): pp. 1331-1336; Feb 2006
  3. Alnouti, Yazen; Petrick, Jay S.; Klaassen, Curtis D.; Tissue Distribution and Ontogeny of Organic Cation Transporters in Mice.; Drug Metabolism and Disposition., 34 (3): pp. 477-482. Mar 2006
  4. Alnouti, Yazen; Shelby, Melinda K.; Chen, Chuan; Klaassen, Curtis D.; Influence of Phenobarbital on Morphine Metabolism and Disposition in the Wistar-Kyoto Rat.; Current Drug Metabolism., 8 (1): pp. 79-89. Jan 2007
  5. Alnouti, Yazen; Klaassen, Curtis D.; Regulation of Sulfotransferase Enzymes by Prototypical Microsomal Enzyme Inducers in Mice.; Journal of Pharmacology and Experimental Therapeutics., 324 (2): pp. 612-621. Feb 2008
  6. Alnouti, Yazen; Bile Acid Sulfation: A Pathway of Bile Acid Elimination and Detoxification. Toxicological Sciences., 108 (2): pp. 225-246. Apr 2009
  7. Huang, J.G.; Bathena, S.P.; Tong, J.; Roth, M.; Hagenbuch, B.; Alnouti, Yazen; Kinetic Analysis of Bile Acid Sulfation by Stably Expressed Human Sulforansferase (SULT2A1). Xenobiotica., 40 (3): pp. 184-194. Mar 2010
  8. Huang, Jiangeng;Bathena, S.P.; Alnouti, Yazen; Metabolite Profiling of Praziquantel and its Analogs During the Analysis of In Vitro Metabolic Stability Using Information-Dependent Acquistion on a Hybrid Triple-Quadrupole Linear Ion Trap Mass Spectrometer.; Drug Metabolism and Pharmacokinetics., 25 (5): pp. 487-499. Sept 2010
  9. Alnouti, Yazen; Klaassen, Curtis D.; Mechanisms of Gender-Specific Regulation of Mouse Sulfotransferases (Sults).; Xenobiotica., 41 (3): pp. 187-97. Mar 2011
  10. Huang, Jiangeng, Bathena, S.P.; Csanaky, Ivan; Alnouti, Yazen; Simultaneous Quantification of Bile Acis and their Sulfate Metabolites in Mouse Liver, Bile, Plasma, Urine Using LC-MS/MS.; Journal of Pharmaceutical and Biomedical Analysis., 55 (5): pp. 1111-1119. Jul 2011