Luis A. Marky, Ph.D.

Professor

Department of Pharmaceutical Sciences
College of Pharmacy
University of Nebraska Medical Center
986025 Nebraska Medical Center
Omaha, NE 68198-6025
402-559-4628 (Office)
402-559-7870/5384 (Lab)
402-559-9543 (Fax)
lmarky@unmc.edu

Teaching Activities:
At the professional level, Dr. Marky lectures in Medicinal Chemistry I where he is the course coordinator, Medicinal Chemistry II, and one elective course "Therapeutic Applications of Proteins and Nucleic Acids".  At the graduate level, Dr. Marky is the course coordinator for Quantitative Pharmaceutical Analysis and Biophysical Chemistry. In addition, he lectures in Advanced Medicinal Chemistry, and Macromolecular Structure and Function. Dr. Marky is a former Fulbright Scholar (Peru-98).

Research Activities/Interests:
The research interests of Dr. Marky are in the area of biophysical chemistry and focus on the molecular forces that control the conformational stability and flexibility of nucleic acids, including Okazaki fragments, hairpin loops, intramolecular triplexes, G-quadruplexes, C-quadruplexes, and three- & four-arm DNA junctions and their interaction with drugs. One objective is to improve the delivery of oligonucleotides into cells and drugs, by using natural and synthetic polymers, to block gene expression. His research group is also investigating the role of water on the conformational stability of biomolecules by examining sequence hydration effects in nucleic acids, drug-nucleic acid complexes, DNA covalent adducts, and protein-DNA interactions.

Recent Publications:

  1. Lee, H.-T.; Carr, C.; Siebler, H.; Waters, L.; Khutsishvili, I.; Iseka, F.; Domack, B.; Olsen, C. M.; Marky, L. A. “A Thermodynamic Approach for the Targeting of Nucleic Acid Structures Using Their Complementary Single Strands” Methods Enzymol. 492, pp. 1-26, 2011. doi: 10.1016/B978-0-12-381268-1.00013-6

  2. Singh, S. K.; Szulik, M.W.; Ganguly, M.; Khutsishvili, I.; Stone, M. P.; Marky, L. A.; Gold, B. “Characterization of DNA with an 8-Oxoguanine Modification” Nucleic Acids Res. 2011, pp. 1–13, 2011. doi: 10.1093/nar/gkr275

  3. Oberoi, H. S.; Laquer, F. C.; Marky, L. A.; Kabanov, A. V.; Bronich, T. K. “Core Cross-linked Block Ionomer Micelles as pH-responsive Carriers for Cis-diamminedichloroplatinum(II)” J. of Controlled Release 153, pp. 64-72, 2011. doi: 10.1016/j.jconrel.2011.03.028

  4. Prakash, A.; Natarajan, A.; Marky, L. A.; Ouellette, M. M.; Borgstahl, G. E. O.  “Stabilization of a G-Quadruplex from Unfolding by Replication Protein A Using Potassium and the Porphyrin TMPyP41” J. Nucleic Acids 2011, pp. 1-13, 2011. doi: 10.4061/2011/529828

  5. Prislan, I.; Khutsishvili, I.; Marky, L. A. “Interaction of Minor Groove Ligands with G-quadruplexes: Thermodynamic Contributions of the Number of quartets, T-U Substitutions, and Conformation” Biochimie 93, pp. 1341-1350, 2011. doi: 10.1016/j.biochi.2011.06.001

  6. Maiti, S.; Kankia, B.; Khutsishvili, I.; Marky, L. A. “Melting Behavior and Ligand Binding of DNA Intramolecular Secondary Structures” Biophysical Chemistry 159, pp. 162-171, 2011. doi: 10.1016/j.bpc.2011.06.006

  7. Prislan, I.; Lee, H.-T.; Lee, C.; Marky, L. A. “The Size of Internal Loops Influences the Unfolding Thermodynamics of DNA Hairpins.” In ACS Symposium Series 1082, R. D. Sheardy and S. A. Winkle, Editors; pp. 93-110, 2011.

  8. Gmeiner, W. H.; Salsbury Jr., F.; Marky, L. A. “The Stability of a Model Substrate for Topoisomerase 1-Mediated DNA Religation Depends on the Presence of Mismatched Base Pairs” J. Nucleic Acids 2011, pp. 1-8, 2011. doi:10.4061/2011/631372.

  9. Kowal, E. A.; Ganguly, M.; Pallan, P. S.; Marky, L. A.; Gold, B.; Egli, M.; Stone, M. P.  “Structure and Dynamics of 7-Deaza-2'-deoxyadenosine in the Dickerson-Drew Dodecamer” J. Physical Chemistry B. 115, pp. 13925-13934, 2011, doi: dx.doi.org/10.1021/jp207 104w.

  10. Lee, H.-T.; Waters, L.; Olsen, C. M.; Khutsishvili, I.; Marky, L. A. “Probing the Temperature Unfolding of a Variety of DNA Secondary Structures Using the Fluorescence Properties of 2-aminopurine” Acta Chimica Slovenica XX, pp. xxx-xxx. 2012, in press.

Luis Marky, Ph.D.
Dr. Marky