Interests pursued in Dr. Hemstreet's laboratory:
- Application of Quantitative Fluorescence Imaging Analysis to the development of a panel of single-cell biomarkers including tissue transglutaminase, Gelsolin, ?-catenin, and E-cadherin for detection of prostate cancer and premalignant disease.
- Application of Quantitative Fluorescence Imaging Analysis to the development of a panel of single-cell biomarkers including DNA ploidy, protein p300, Gelsolin, survivin, and BLCA-4 for detection of bladder cancer and premalignant disease.
- Quantitative Fluorescence Imaging Analysis of ARF, Mdm2, and p53 comprising a Myc-induced apoptotic pathway, as potential biomarkers of prostate cancer and premalignant disease, and bladder cancer and premalignancy.
- Development of fluorescent nanocrystal technology for quantitative analysis of multiplexed signals produced in single cells and representing panels of protein biomarkers of prostate cancer and premalignant disease, and bladder cancer and premalignancy.
- Quantitative Fluorescence Imaging Analysis of oxidative stress markers including protein carbonyls, hydroxynonenal, malondialdehyde, 8-oxoguanine, and estrogen quinone in single cells collected from cancer-bearing, benign hyperplastic, and normal prostate glands.
- Optimization of tissue processing procedures including fixation, cryopreservation, paraffin embedding, and live tissue sectioning for quantitative measurements of single-cell biomarkers of prostate cancer and premalignant disease, and studies of these same biomarkers in micro-organ culture.