To schedule appointments please review the Cell Analysis Facility instrument calendars on the Research Support System Core Facilities web-portal (requires log-in with Outlook ID and password) and then call the facility personnel to make an appointment 402-559-6267. Our knowledgeable staff will discuss details related to your project and work with you to reserve time on an appropriate instrument for your experiment. Additionally, the staff are available for advice and consultation on which fluorochromes and antibodies may suit your needs.
Costs related to the project will be determined by the number of samples you intend to analyze and the type of experiment you are proposing. Experiments are scheduled on a first come first served basis. Unscheduled walk-in experiments will be accommodated as time allows, but please be aware that frequently means an operator must stay late and your project will be subject to after business hours rate if that is the only time available. Sorters are often reserved 1-3 weeks in advance due to high demand, please plan ahead. We ask that you give us 24 hour notice to cancel an appointment so that we may offer that time to other investigators, cancellations with less than 24 hour notice for sorting experiments may be subject to a fee.
All samples are to be fixed unless there is a biological reason that precludes fixation. You must disclose to facility personnel if you intend to use unfixed samples prior to scheduling the use of facility resources.
Sorting time is billed from the time that the instrument was reserved for your use until it is available for another investigator's use, this does include time spent waiting while additional cell lines are prepared, while your staff leaves for lunch, time spent assessing the feasibility of a particular project or any other reason that a sorter is reserved for you and unavailable to other users. Please be respectful of these constraints and do not reserve time that you do not intend to use.
You must have a UNMC cost object or other payment method registered with the facility in order to contract services. Your designated account will be automatically debited at the end of each month for services provided during that billing period. You will receive a courtesy notice when your account has been debited, billing statements and pending charges can be viewed through the Research Support System for Core Facilities at any time.
Frequently Asked Questions
What do I need to bring for a sorting appointment?
- Bring your cost center number
- Your cells of interest in PBS, preferably in counts of 5 million cells/ml of PBS.
- Collection tubes with media. Collection tubes can be 12x75 mm or 15ml conical tubes. Each tube should have approximately 1ml of media. The 12x75 mm tubes can hold approximately 600,000 cells while the 15ml tubes can hold up to 1.5 million sorted cells.
- Bring a negative control with cells that have been treated in the same manner as the cells to be sorted.
- If the sort is multicolor, bring compensation controls. Compensation controls must include tubes with cells that have been treated in the same manner as cells to be sorted. There should be the same number of compensation tubes as there are fluorochromes in the sort. Each tube should have cells and single antibody staining with an antibody for which the cells will show positive expression. SINGLE COLOR EXPERIMENTS ONLY REQUIRE A NEGATIVE CONTROL.
What do I need to bring for an experiment on the Calibur, LSR or Array?
- Bring your cost center number and the experimental layout (plate or tube).
- Bring a negative control with cells that have been treated in the same manner as the cells to be analyzed.
- If the experiment is multicolor, bring compensation controls. Compensation controls must include tubes with cells that have been treated in the same manner as the cells to be analyzed. There should be the same number of compensation tubes as there are fluorochromes. Each tube should have cells (or Comp beads) and a single antibody for which the cells will show positive expression. SINGLE COLOR EXPERIMENTS ONLY REQUIRE A NEGATIVE CONTROL.
What number do I call if I want to schedule an appointment?
402-559-6267. Tell lab personnel what you are bringing, what you want done to them, and how many samples.
Who do I contact if I have a new experiment and I am unsure how to proceed or I have general questions?
Consultations can be scheduled with Charlie Kuszynski, PhD by email or by phone 402-559-6299; and Flow Cytometrist, Victoria Smith by email or at 402-559-6267.
Consultations can be done over the phone or in person. If meeting in person please bring any references that you are using to the meeting.
What notice do I have to give if I want to bring samples to the lab to be run?
Appointments should be scheduled as far in advance as possible, please review the on-line instrument calendar for instrument availability. While appointments can be made the day of the experiment, they are made on a first come first served basis. If it is imperative that results are ready by a certain time plan ahead and give at least a day’s notice. The cell sorter appointments can fill up to a week in advance, so plan ahead and call as soon as you think you will need one.
What do I do if I need to cancel an appointment?
Call the lab at 402-559-6267 and let someone know you will be unable to make the appointment.
Sort appointments must be canceled prior to 10:00 AM on the day of the sort to avoid a $45 setup fee.
Research Support Offices
- Clinical Research Resources
- Nebraska Biobank
Grant Writing Support
Centers and Major Programs
- Sponsored Programs Administration (SPA)
- Government Relations
- Intellectual Property Assistance
VCR Managed Core Facilities
- Cell Analysis Facility
- Mass Spectrometry and Proteomics Core Facility
- Core Facility Scheduling and Billing