New Users

We are improving parts of our website, this information is under construction. If you are new to the facility, please contact us for further information. 

Flow Analysis and Cell Sorting
Includes set up and processing of specimens

  • Immunofluorescence (1-15 colors, 1-17 parameters)
  • Surface expression of standard CD markers
  • Specialized expression of surface markers with custom antibodies
  • Gene transduction/expression
  • Green, yellow, red and cyan fluorescent proteins
  • Receptor expression
  • Apoptosis (A0 determination)
  • DNA analysis
  • Cell cycle assays
  • Cancer therapy
  • Kinetic studies (time dependent assay)
  • Multiplex bead assay
  • Cell sorting, enrichment, purification
  • Single cell cloning, high speed
  • AutoMACS - magnetic separation for pre-sort sample enrichment
  • New protocol development

Other Services

  • Consultations for experiment development, data interpretation and troubleshooting
  • Extensive protocol library for flow cytometry techniques
  • Training in theory and practice of flow cytometry
  • Data collection and analysis of user provided samples
  • Advice on tissue or cell preparation and appropriate use of fluorophores
  • Training in use of “offline” list mode data analysis software
  • Data interpretation
  • Publication quality graphics

Questions?

402-559-6267

Philip Hexley, Ph.D., Director
Email

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