Recommended Sample Amounts for Mass Spectrometric Analysis*

Type of Service Instrument Minimum Amount Optimal Amount** Notes
Protein Identification  5600 TripleTOF  15 fmol  500 fmol Coomassie Blue stained gel bands or in solution.
LTQ/Velos   15 fmol 500 fmol 
4800 MALDI  100 fmol 500 fmol 
Protein Mass 4800 MALDI 100 fmol 1 pmol Concentrations above 1 pmol/µL in volatile solvents free of non-volatile buffers, detergents, salts, urea, glycerol, etc. Suitable solvents are water, acetonitrile/water or methanol/water mixtures or dilute, volatile buffers (e.g., 1 mM NH4HCO3).
Peptide Mass   4800 MALDI 100 fmol  1 pmol 

Concentrations above 1 pmol/µL in volatile solvents free of non-volatile buffers, detergents, salts, urea, glycerol, etc. Suitable solvents are water, acetonitrile/water or methanol/water mixtures or dilute, volatile buffers (e.g., 1 mM NH4HCO3).

*After tryptic digest and sample processing, expected recovery is 45-50%, but can be as low as 30%.  This must be taken into account when submitting samples.

**Based on well ionized peptides

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