David Klinkebiel

Assistant Professor, Biochemistry and Molecular Biology, and Director, Epigenomics Core Facility

Dave Klinkebiel, PhDPhone: 402-559-3842 (Office)
    Location: BCC 10.12.396
Fax: 402-559-6650

View the Epigenomics Core Facility homepage.

Ph.D., University of Nebraska-Lincoln, 1990


Research Interest:
Epigenomics – Influence of DNA methylation and histone modification on gene expression and the association with cancer development.

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Toure D.M., Baccaglini L., Opoku S.T., Barnes-Josiah D., Cox R., Hartman T., Klinkebiel D., Epigenetic Dysregulation of Insulin-like Growth Factor (IGF)-related Genes and Adverse Pregnancy Outcomes: A Systematic Review. J Matern Fetal Neonatal Med. 2016 Jan 8:1-26. PMID:26743165

Haney S.L., Upchurch G.M., Opavska J., Klinkebiel D., Hlady R.A., Suresh A., Pirruccello, S.J., Shukla V., Lu R., Costinean S., Rizzino A.,  Karpf A.R., Joshi S., Swanson P., Opavsky R. Promoter Hypomethylation and Expression Is Conserved in Mouse Chronic Lymphocytic Leukemia Induced by Decreased or Inactivated Dnmt3a. Cell Reports, 2016, Volume 15, Issue 6, p1190–1201, DOI: Article

Klinkebiel, D., Zhang, W., Akers, S., Odunsi, K., and A. R. Karpf. DNA methylome analyses implicate fallopian tube as the tissue of origin for high-grade serous ovarian cancer. Mol Cancer Res. 2016 Sep;14(9):787-94. doi: 10.1158/1541-7786.MCR-16-0097. Epub 2016 Jun 3. PMID: 27259716

Zhang W., Barger C.J., Eng K.H., Klinkebiel D., Link P.A., Omilian A., Bshara W., Odunsi K., Karpf A.R., PRAME expression and promoter hypomethylation in epithelial ovarian cancer. Oncotarget. 2016 Jun 13. doi: 10.18632/oncotarget.9977. PMID: 27322684

Rajendiran S., Gibbs L.D., Van Treuren T., Klinkebiel D.L., Vishwanatha J.K., MIEN1 is tightly regulated by SINE Alu methylation in its promoter. Oncotarget. 2016 Aug 29. doi: 10.18632/oncotarget.11675. [Epub ahead of print] PMID: 27589566

Haney SL, Upchurch GM, Opavska J, Klinkebiel D, Appiah AK, Smith LM, Heavican TB, Iqbal J, Joshi S, Opavsky R. Loss of Dnmt3a induces CLL and PTCL with distinct methylomes and transcriptomes in mice. Sci Rep. 2016 Sep 28;6:34222. doi: 10.1038/srep34222. PMID: 27677595

Haney SL, Upchurch GM, Opavska J, Klinkebiel D, Hlady RA, Roy S, Dutta S, Datta K, Opavsky R. Dnmt3a Is a Haploinsufficient Tumor Suppressor in CD8+ Peripheral T Cell Lymphoma. PLoS Genet. 2016 Sep 30;12(9):e1006334. doi: 10.1371/journal.pgen.1006334. eCollection 2016 Sep. PMID: 27690235

Yang, J.L., Klinkebiel. D., Boland. M.J., Tang. L., Christman. J.K., 2005. Promoter methylation and mRNA expression of MCF10 model cell lines of breast cancer. Chinese Journal of Pathology. Zhonghua Bing Li Xue Za Zhi. March;34(3):177-8.

Yang, J.L., Klinkebiel, D., Boland, M.J., Tang, L., Christman, J.K., 2006. Promoter methylation and mRNA expression of APC gene in MCF10 breast cancer model. Chinese Journal of Pathology. Zhonghua Bing Li Xue Za Zhi. Jan;35(1):32-6. Abstract

Yang, J.L., Klinkebiel, D., Boland, M.J., Tang, L., Christman, J.K. 2007. Promoter methylation and mRNA expression of WT1 gene in MCF10 breast cancer model. Chinese Journal of Pathology. Zhonghua Bing Li Xue Za Zhi. Apr;36(4):253-8. Abstract

Radhakrishnan, P., Basma, H., Klinkebiel, D., Christman, J., Cheng, P.W. 2008 Cell type-specific activation of the cytomegalovirus promoter by dimethyl sulfoxide and 5-Aza-2'-deoxycytidine. Int. J. Biochem. Cell Biol. 40(9)1944-55. Abstract

Chew YC, West JT, Kratzer SJ, Ilvarsonn AM, Eissenberg JC, Dave BJ, Klinkebiel D, Christman JK, Zempleni J. Biotinylation of histones represses transposable elements in human and mouse cells and cell lines and in Drosophila melanogaster. J Nutr. 138(12):2316-22, 2008. Abstract

Iqbal J, Kucuk C, Deleeuw RJ, Srivastava G, Tam W, Geng H, Klinkebiel D, Christman JK, Patel K, Cao K, Shen L, Dybkaer K, Tsui IF, Ali H, Shimizu N, Au WY, Lam WL, Chan WC. Genomic analyses reveal global functional alterations that promote tumor growth and novel tumor suppressor genes in natural killer-cell malignancies. Leukemia 23:1139-51. Abstract

Chachadi V.B., Cheng H., Klinkebiel D., Christman J.K., Cheng P.W. 5-Aza-2'-deoxycytidine Increases Sialyl Lewis X on MUC1 by Stimulating β-Galactoside α2,3-Sialyltransferase 6 Gene. 2011. Int J Biochem Cell Biol. 43(4):586-93

Hlady R., Novakova S., Opavska J., Klinkebiel D., Peters S.L., Bies J., Hannah J., Iqbal J., Anderson K.A., Siebler H.M., Smith L.M., Greiner T.C., Bastola D., Joshi S., Lockridge O., Simpson M.A., Felsher D.W., Wagner K., Chan W.C., Christman J.K., and Opavsky R., Loss of Dnmt3b function upregulates the tumor modifier Ment and accelerates mouse lymphomagenesis. 2012. J. of Clinical Investigation. 122(1):163–177.

Küçük C., Hu X., Iqbal J., Gaulard P., Klinkebiel D., Cornish A., Dave B.J., and Chan W.C. HACE1 is a tumor suppressor gene candidate in natural killer cell neoplasms. 2013. American Journal of Pathology 182(1):49-55.

Tian C., Liu Q., Ma K., Wang Y., Chen Q., Ambroz R., Klinkebiel D.L., Li Y., Huang Y., Ding J., Wu J. and Zheng J.C. Characterization of Induced Neural Progenitors from Skin Fibroblasts by a Novel Combination of Defined Factors. 2013, Nature-Scientific Reports. 3, 1345.

Peters S.L., Hlady R.A., Opavska J., Klinkebiel D., Novakova S., Smith L.M., Lewis R.E., Karpf A.R., Simpson M.A., Wu L., Opavsky R. An essential role for Dnmt1 in the prevention and maintenance of MYC-induced T-cell lymphomas. Molecular Cell Biology. 2013 Nov;33(21):4321-33.

Peters S.L., Hlady R.A., Opavska J., Klinkebiel D., Pirruccello S.J., Talmon G.A., Sharp J.G., Wu L., Jaenisch R., Simpson M.A., Karpf A.R., Opavsky R. Tumor suppressor functions of Dnmt3a and Dnmt3b in the prevention of malignant mouse lymphopoiesis. Leukemia. 2014 May;28(5):1138-42.

Hu X., Kucuk C., Jiang B., Klinkebiel D., Geng H., Gong Q., Bouska A., Iqbal .J, Gaulard P., McKeithan T.W., Chan W.C. Global promoter methylation analysis reveals novel candidate tumor suppressor genes in natural killer cell lymphoma. Clin Cancer Res. 2015 Jan 22.

Haney S.L., Hlady R.A., Opavska J., Klinkebiel D., Pirruccello S.J., Dutta S., Datta K., Simpson M.A., Wu L., Opavsky R. Methylation-independent repression of Dnmt3b contributes to oncogenic activity of Dnmt3a in mouse MYC-induced T-cell lymphomagenesis. Oncogene. 2015 Feb 2. doi: 10.1038/onc.2014.472.

Current Funding:

Nebraska Research Initiative - Core Facility Program
Epigenomics Core Facility, UNMC