Robert F. Ramaley

Emeritus Professor, Biochemistry and Molecular Biology

Robert Ramaley, PhDPhone: 402-559-6662
Fax: 402-559-6650

Ph.D., University of Minnesota, 1964

Primary Research/Clinical Interests/Expertise:
Biochemistry; Biology, Developmental/Evolutionary; Biotechnology; Brain; Computer Graphics; Computer Modeling; Environmental Health; Enzymology; Gene Cloning; Metabolic Diseases; Microbiology; Recombinant DNA;Thermostable Enzymes;Thermophile Microorganisms

Studies in the laboratory center around the purification and structure/function studies of enzymes and proteins. These studies include the cloning and expression of developmentally regulated genes involved in bacterial sporulation, the enzymes involved in microbial pathogenicity and the enzymes and proteins from extremely thermophilic bacteria. Current collaborative studies are being conducted on a new method for the purification of human alpha-fetoprotein and the large scale purification of HIV-protease. 

Most Significant Publications:

Vasantha, N., B. Uratini, R. F. Ramaley and E. Freese. Isolation of a Development Gene of Bacillus subtilis and its Expression in Escherichia coli. Proc. Natl. Acad. Sci. 80:785-789 (1983).

Ramaley, R.F. and N. Vasantha. Glycerol Protection and Purification of Bacillus subtilis Glucose Dehydrogenase. J. Biol. Chem. 258:12558-12565 (1983).

Ebadi, M., S. Wilt, R. Ramaley, S. Swanson and C. Mebus. The Role of Zinc and Zinc-Binding Proteins in Regulation of Glutamic Acid Decarboxylase in Brain. in: Chemical and Biological Aspects of Vitamin B6 Catalysis, A.E. Evangelopoulos, ed., part A. pp. 255-275 (1984) A.R. Liss Inc. N.Y.

Lampel, K.A., B. Uratani, G.R. Chaudhry, R. F. Ramaley and S. Rudikoff. Characterization of the Developmentally-Regulated Bacillus subtilis Glucose Dehydrogenase Gene. J. Bacteriol. 166:238-243 (1986).

Smith, E.P. and R. F. Ramaley. An Improved Method for the Purification of Bacillus subtilis Glucose Dehydrogenase Cloned in Escherichia coli. Prep. Biochem. 18:165-182 (1988).

Lane, E.M., R. F. Ramaley and H.G. Miller. Hemolysin Production by Leptospira interrogans Serovar Canicola in a Protein-Free Medium with Hemin. Southeast Asian J. Trop. Med. Pub. Hlth. 19:187-190 (1988).

Lane, E.M., R. F. Ramaley and H.G. Miller. Variations in the Hemolytic Activity of Leptospira interrogans serovar Canicola. Southeast Asian J. Trop. Med. Pub. Hlth. 19:629-634 (1988).

Doerr, C.A., R. F. Ramaley, M.C. Haven and B.A. Buehler. Separation of Human Alpha-Fetoprotein from Human Albumin Using Hydrophobic Column Chromatography. Bio. Chrom. 4:69-73 (1989).

Chudomelka, P.J., R.F. Ramaley and L.C. Murrin. Histidine Decarboxylase from Rat and Rabbit Brain: Partial Purification and Characterization. Neurochem. Res. 15:17-24 (1990).

Nagarajan, V., R. Ramaley, H. Albertson and M. Chen. Secretion of Streptavidin from Bacillus subtilis. Applied and Environmental Microbiology 59:3894-3898 (1993). 

Rothermund, C. and R. Ramaley. Electron Micrograph of Moorella Obsidium. in: One Universe: At Home in the Cosmos. N. DeGrasse Tyson, C. Liu and R. Irion, eds. Rose Center for Earth and Space, American Museum of Natural History, Joseph Henry Press, Washington, D.C., p. 160 (1999).

Ramaley, R.F., P.L. Scanlan and W. O'Dell. Presence of Thermophilic Nagleria Isolates in the Yellowstone and Grand Teton National Parks. in: Thermophiles: Biodiversity, Ecology and Evolution. A.L. Reysenbach, M. Voytek and R. Mancinelli, eds., pp. 41-50 (2001). Kluwer Academic/Plenum Publisher Press, New York.

Lindstrom, R.F., R.F. Ramaley, and R.L. Bizzoco. Invisible Invasion: Potential Contamination of Yellowstone Hot Springs by Human Activity. Western North America Naturalist 62:44-58 (2002).